基于蒙特卡洛法的从混浊介质中提取固有荧光的模型。
Monte-Carlo-based model for the extraction of intrinsic fluorescence from turbid media.
作者信息
Palmer Gregory M, Ramanujam Nirmala
机构信息
Duke University, Department of Radiation Oncology, Box 3455, Durham, North Carolina 27710, USA.
出版信息
J Biomed Opt. 2008 Mar-Apr;13(2):024017. doi: 10.1117/1.2907161.
Abstract
A Monte-Carlo-based model of fluorescence is developed that is capable of extracting the intrinsic fluorescence properties of tissue, which are independent of the absorption and scattering properties of tissue. This model is flexible in its applicability to different illumination-collection geometries and is also valid for a wide range of optical properties, representative of tissue in the UV-visible spectrum. This is potentially useful in a variety of biomedical applications, including cancer diagnostics and monitoring the physiological response to therapy. The model is validated using phantoms composed of hemoglobin (absorber), polystyrene spheres (scatterer), and furan-2 (fluorophore). It is found that this model is able to retrieve the intrinsic fluorescence spectra of the tissue phantoms and recover the intrinsic fluorescence intensity of furan within the phantoms to within a mean error of less than 10%.
摘要
开发了一种基于蒙特卡罗的荧光模型，该模型能够提取组织的固有荧光特性，这些特性与组织的吸收和散射特性无关。该模型在应用于不同的照明-采集几何结构时具有灵活性，并且对于代表紫外-可见光谱中组织的广泛光学特性也有效。这在各种生物医学应用中可能有用，包括癌症诊断和监测对治疗的生理反应。使用由血红蛋白（吸收剂）、聚苯乙烯球体（散射体）和呋喃-2（荧光团）组成的体模对该模型进行了验证。结果发现，该模型能够检索组织体模的固有荧光光谱，并将体模内呋喃的固有荧光强度恢复到平均误差小于10%的范围内。
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