三价氮氧自由基作为独特的分子探针，可同时测量氧化还原状态和氧合作用。
Trityl-nitroxide biradicals as unique molecular probes for the simultaneous measurement of redox status and oxygenation.
机构信息
Center for Biomedical EPR Spectroscopy and Imaging, The Davis Heart and Lung Research Institute, The Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
出版信息
Chem Commun (Camb). 2010 Jan 28;46(4):628-30. doi: 10.1039/b919279d. Epub 2009 Nov 18.
Abstract
Novel trityl-nitroxide biradicals were synthesized and exhibited enhanced sensitivity and stability for rapid and simultaneous measurement of redox status and oxygenation by electron paramagnetic resonance spectroscopy.
摘要
新型三价氮氧自由基双自由基被合成出来，并通过电子顺磁共振波谱法显示出增强的敏感性和稳定性，可用于快速和同时测量氧化还原状态和氧合作用。
相似文献
1
Trityl-nitroxide biradicals as unique molecular probes for the simultaneous measurement of redox status and oxygenation.三价氮氧自由基作为独特的分子探针，可同时测量氧化还原状态和氧合作用。
Chem Commun (Camb). 2010 Jan 28;46(4):628-30. doi: 10.1039/b919279d. Epub 2009 Nov 18.
2
Synthesis of 14N- and 15N-labeled trityl-nitroxide biradicals with strong spin-spin interaction and improved sensitivity to redox status and oxygen.合成具有强自旋-自旋相互作用和对氧化还原状态及氧灵敏度提高的 14N-和 15N 标记三苯甲基氮氧自由基双自由基。
J Org Chem. 2010 Nov 19;75(22):7796-802. doi: 10.1021/jo1016844. Epub 2010 Oct 28.
3
Supramolecular host-guest interaction of trityl-nitroxide biradicals with cyclodextrins: modulation of spin-spin interaction and redox sensitivity.三苯甲基-氮氧双自由基与环糊精的超分子主客体相互作用：自旋-自旋相互作用和氧化还原敏感性的调节
Org Biomol Chem. 2016 Feb 7;14(5):1694-701. doi: 10.1039/c5ob02450a.
4
Synthesis of trityl radical-conjugated disulfide biradicals for measurement of thiol concentration.三苯甲基自由基共轭双二硫自由基的合成及其用于巯基浓度的测定。
J Org Chem. 2011 May 20;76(10):3853-60. doi: 10.1021/jo200265u. Epub 2011 Apr 29.
5
Designing Molecular Probes To Prolong Intracellular Retention: Application to Nitroxide Spin Probes.设计用于延长细胞内保留时间的分子探针：应用于氮氧化物自旋探针。
Bioconjug Chem. 2016 Dec 21;27(12):2923-2930. doi: 10.1021/acs.bioconjchem.6b00595. Epub 2016 Dec 1.
6
Effect of Solution Ionic Strength on the pK of the Nitroxide pH EPR Probe 2,2,3,4,5,5-Hexamethylimidazolidin-1-oxyl.溶液离子强度对氮氧化物pH电子顺磁共振探针2,2,3,4,5,5-六甲基咪唑啉-1-氧基pK的影响
Cell Biochem Biophys. 2017 Jun;75(2):185-193. doi: 10.1007/s12013-017-0780-y. Epub 2017 Feb 17.
7
Novel ferrocenyl nitroxide nanoparticles as electron paramagnetic resonance oximetry probes in vitro and in vivo.新型二茂铁基氮氧自由基纳米颗粒作为体外和体内电子顺磁共振血氧测定探针。
Nanomedicine (Lond). 2011 Feb;6(2):225-31. doi: 10.2217/nnm.11.2.
8
Synthesis and properties of novel porphyrin spin probes containing isoindoline nitroxides.含异吲哚啉氮氧化物的新型卟啉自旋探针的合成与性质
Free Radic Biol Med. 2007 Jul 1;43(1):111-6. doi: 10.1016/j.freeradbiomed.2007.03.032. Epub 2007 Apr 10.
9
Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe.使用不依赖序列的氮氧自由基探针进行核酸中纳米距离的定点自旋标记测量。
Nucleic Acids Res. 2006;34(17):4722-30. doi: 10.1093/nar/gkl546. Epub 2006 Sep 10.
10
Three-dimensional electron paramagnetic resonance imaging of mice using ascorbic acid sensitive nitroxide imaging probes.使用抗坏血酸敏感的氮氧化物成像探针进行小鼠的三维电子顺磁共振成像。
Free Radic Res. 2021 Oct;55(9-10):950-957. doi: 10.1080/10715762.2021.1991918. Epub 2021 Oct 25.
引用本文的文献
1
Sensitive simultaneous measurements of oxygenation and extracellular pH by EPR using a stable monophosphonated trityl radical and lithium phthalocyanine.通过使用稳定的单膦化三苯甲基自由基和酞菁锂的电子顺磁共振（EPR）技术对氧合作用和细胞外 pH 值进行敏感的同时测量。
Free Radic Biol Med. 2024 Mar;213:11-18. doi: 10.1016/j.freeradbiomed.2024.01.012. Epub 2024 Jan 11.
2
An EPR Study on Highly Stable Nitroxyl-Nitroxyl Biradicals for Dynamic Nuclear Polarization Applications at High Magnetic Fields.高磁场下用于动态核极化应用的高稳定氮氧自由基-氮氧自由基双自由基的 EPR 研究。
Molecules. 2023 Feb 17;28(4):1926. doi: 10.3390/molecules28041926.
3
Highly bioresistant, hydrophilic and rigidly linked trityl-nitroxide biradicals for cellular high-field dynamic nuclear polarization.用于细胞高场动态核极化的具有高度生物抗性、亲水性且刚性连接的三苯甲基-氮氧化物双自由基。
Chem Sci. 2022 Nov 17;13(47):14157-14164. doi: 10.1039/d2sc04668g. eCollection 2022 Dec 7.
4
Synthesis and characterization of a Cu(ii) coordination-containing TAM radical as a nitroxyl probe.一种含铜（II）配位的TAM自由基作为硝酰基探针的合成与表征
RSC Adv. 2022 May 27;12(25):15980-15985. doi: 10.1039/d1ra07511j. eCollection 2022 May 23.
5
Large-scale synthesis of a monophosphonated tetrathiatriarylmethyl spin probe for concurrent measurement of O, pH and inorganic phosphate by EPR.用于通过电子顺磁共振同时测量氧、pH值和无机磷酸盐的单膦酸化四硫代三芳基甲基自旋探针的大规模合成。
RSC Adv. 2021 Jul 27;11(42):25951-25954. doi: 10.1039/d1ra04551b. eCollection 2021.
6
Biological Applications of Electron Paramagnetic Resonance Viscometry Using a C-Labeled Trityl Spin Probe.使用 C 标记的三苯甲基自旋探针的电子顺磁共振粘度法的生物学应用。
Molecules. 2021 May 8;26(9):2781. doi: 10.3390/molecules26092781.
7
A C-Labeled Triarylmethyl Radical as an EPR Spin Probe Highly Sensitive to Molecular Tumbling.一种 C 标记的三芳基甲基自由基作为 EPR 自旋探针，对分子滚转高度敏感。
Angew Chem Int Ed Engl. 2020 Sep 14;59(38):16451-16454. doi: 10.1002/anie.202006591. Epub 2020 Jul 21.
8
Synthesis of hydroxyethyl tetrathiatriarylmethyl radicals OX063 and OX071.羟乙基四硫代三芳基甲基自由基OX063和OX071的合成。
RSC Adv. 2019;9(60):35073-35076. doi: 10.1039/c9ra08633a. Epub 2019 Oct 30.
9
Diastereoisomers of l-proline-linked trityl-nitroxide biradicals: synthesis and effect of chiral configurations on exchange interactions.L-脯氨酸连接的三苯甲基-氮氧双自由基的非对映异构体：合成及手性构型对交换相互作用的影响
Chem Sci. 2018 Apr 5;9(19):4381-4391. doi: 10.1039/c8sc00969d. eCollection 2018 May 21.
10
Efficient cross-effect dynamic nuclear polarization without depolarization in high-resolution MAS NMR.高分辨率魔角旋转核磁共振中无退极化的高效交叉效应动态核极化
Chem Sci. 2017 Dec 1;8(12):8150-8163. doi: 10.1039/c7sc02199b. Epub 2017 Oct 2.
本文引用的文献
1
Trityl-based EPR probe with enhanced sensitivity to oxygen.对氧气具有增强灵敏度的基于三芳基甲基的电子顺磁共振探针。
Free Radic Biol Med. 2009 Sep 1;47(5):654-8. doi: 10.1016/j.freeradbiomed.2009.06.007. Epub 2009 Jun 10.
2
Synthesis of a BDPA-TEMPO biradical.BDPA-TEMPO双自由基的合成。
Org Lett. 2009 May 7;11(9):1871-4. doi: 10.1021/ol9001575.
3
Esterified trityl radicals as intracellular oxygen probes.酯化三苯甲基自由基作为细胞内氧探针。
Free Radic Biol Med. 2009 Apr 1;46(7):876-83. doi: 10.1016/j.freeradbiomed.2008.12.011. Epub 2008 Dec 24.
4
Highly stable dendritic trityl radicals as oxygen and pH probe.高度稳定的树枝状三苯甲基自由基作为氧和pH探针。
Chem Commun (Camb). 2008 Sep 28(36):4336-8. doi: 10.1039/b807406b. Epub 2008 Aug 15.
5
In vivo EPR measurement of glutathione in tumor-bearing mice using improved disulfide biradical probe.使用改进的二硫双自由基探针在荷瘤小鼠体内进行谷胱甘肽的电子顺磁共振测量。
Free Radic Biol Med. 2008 Aug 1;45(3):312-20. doi: 10.1016/j.freeradbiomed.2008.04.019. Epub 2008 Apr 23.
6
Synthesis and characterization of ester-derivatized tetrathiatriarylmethyl radicals as intracellular oxygen probes.酯衍生化四硫代三芳基甲基自由基作为细胞内氧探针的合成与表征
J Org Chem. 2008 Feb 15;73(4):1490-7. doi: 10.1021/jo7022747. Epub 2008 Jan 18.
7
Use of electron paramagnetic resonance spectroscopy to evaluate the redox state in vivo.利用电子顺磁共振波谱法评估体内氧化还原状态。
Antioxid Redox Signal. 2007 Oct;9(10):1757-71. doi: 10.1089/ars.2007.1718.
8
Probing the intracellular redox status of tumors with magnetic resonance imaging and redox-sensitive contrast agents.利用磁共振成像和氧化还原敏感型造影剂探究肿瘤细胞内的氧化还原状态。
Cancer Res. 2006 Oct 15;66(20):9921-8. doi: 10.1158/0008-5472.CAN-06-0879.
9
TOTAPOL: a biradical polarizing agent for dynamic nuclear polarization experiments in aqueous media.TOTAPOL：一种用于水介质中动态核极化实验的双自由基极化剂。
J Am Chem Soc. 2006 Sep 6;128(35):11385-90. doi: 10.1021/ja061284b.
10
Simultaneous molecular imaging of redox reactions monitored by Overhauser-enhanced MRI with 14N- and 15N-labeled nitroxyl radicals.通过奥弗豪泽增强磁共振成像（Overhauser-enhanced MRI）利用14N和15N标记的氮氧自由基对氧化还原反应进行同步分子成像。
Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1463-8. doi: 10.1073/pnas.0510670103. Epub 2006 Jan 23.
Zotero 插件