Gluth Teresa D, Poncelet Martin, DeVience Stephen, Gencheva Marieta, Hoblitzell Emily H, Khramtsov Valery V, Eubank Timothy D, Driesschaert Benoit
Department of Pharmaceutical Sciences, West Virginia University, School of Pharmacy Morgantown WV 26506 USA
In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Sciences Center, West Virginia University Morgantown WV 26506 USA.
RSC Adv. 2021 Jul 27;11(42):25951-25954. doi: 10.1039/d1ra04551b. eCollection 2021.
Low-field electron paramagnetic resonance spectroscopy paired with , a mono-phosphonated triarylmethyl radical, is an unmatched technique for concurrent and non-invasive measurement of oxygen concentration, pH, and inorganic phosphate concentration for investigations. However, the prior reported synthesis is limited by its low yield and poor scalability, making wide-spread application of unfeasible. Here, we report a new strategy for the synthesis of with significantly greater yields demonstrated on a large scale. We also present a standalone application with user-friendly interface for automatic spectrum fitting and extraction of O, pH, and [P] values. Finally, we confirm that remains in the extracellular space and has low cytotoxicity appropriate for local injection.
低场电子顺磁共振光谱与一种单膦化三芳基甲基自由基相结合，是用于同时进行非侵入性测量氧浓度、pH值和无机磷酸盐浓度的无与伦比的技术，适用于相关研究。然而，先前报道的合成方法受限于其低产率和较差的可扩展性，使得该自由基的广泛应用变得不可行。在此，我们报告了一种合成该自由基的新策略，该策略在大规模制备时具有显著更高的产率。我们还展示了一个具有用户友好界面的独立应用程序，用于自动光谱拟合以及提取氧、pH值和磷酸盐浓度值。最后，我们证实该自由基保留在细胞外空间，并且具有适合局部注射的低细胞毒性。
