Food Composition Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
Anal Bioanal Chem. 2010 May;397(2):471-81. doi: 10.1007/s00216-009-3373-9. Epub 2010 Jan 10.
Assessing dietary intake of vitamins from all sources, including foods, dietary supplements, and fortified foods, would be aided considerably by having analytical methodologies that are capable of simultaneous determination of several vitamins. Vitamins naturally present in foods may occur in different chemical forms, with levels ranging over several orders of magnitude. Vitamins in dietary supplements and fortified foods, however, are typically added in a single chemical form, and matrix issues are usually not as complex. These sources should thus be relatively amenable to approaches that aim for simultaneous determination of multiple vitamins. Our recent work has focused on development of liquid chromatography (LC)-UV/fluorescence and LC-tandem mass spectrometry methods for the simultaneous determination of water-soluble vitamins (thiamine, niacin, pyridoxine, pantothenic acid, folic acid, biotin, and riboflavin) in dietary supplement tablets and fortified foods, such as formula powders and breakfast cereals. As part of the validation of our methods and collaboration in characterization of a new NIST SRM 1849 Infant/Adult Nutritional Formula powder, we report data on SRM 1849 using isotope dilution mass spectrometric methods. Use of available NIST Standard Reference Materials(R) as test matrices in our method development and validation gives a benchmark for future application of these methods. We compare three chromatographic approaches and provide data on stability of vitamin standard solutions for LC-based multiple vitamin determinations.
评估来自所有来源（包括食物、膳食补充剂和强化食品）的维生素摄入量，如果有能够同时测定多种维生素的分析方法，将会得到极大的帮助。食物中天然存在的维生素可能以不同的化学形式存在，其水平跨越几个数量级。然而，膳食补充剂和强化食品中的维生素通常以单一的化学形式添加，基质问题通常不那么复杂。因此，这些来源应该相对容易采用旨在同时测定多种维生素的方法。我们最近的工作重点是开发液相色谱（LC）-UV/荧光和 LC-串联质谱法，用于同时测定膳食补充片剂和强化食品（如配方粉和早餐麦片）中的水溶性维生素（硫胺素、烟酸、吡哆醇、泛酸、叶酸、生物素和核黄素）。作为我们方法验证和参与表征新的 NIST SRM 1849 婴儿/成人营养配方粉工作的一部分，我们报告了使用同位素稀释质谱法测定 SRM 1849 的数据。在我们的方法开发和验证中使用现有的 NIST 标准参考物质（R）作为测试基质，为未来应用这些方法提供了基准。我们比较了三种色谱方法，并提供了基于 LC 的多种维生素测定中维生素标准溶液稳定性的数据。
