Phillips Melissa M
Chemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8392, USA,
Anal Bioanal Chem. 2015 Apr;407(11):2965-74. doi: 10.1007/s00216-014-8354-y. Epub 2014 Nov 30.
Vitamins are essential for improving and maintaining human health, and the main source of vitamins is the diet. Measurement of the quantities of water-soluble vitamins in common food materials is important to understand the impact of vitamin intake on human health, and also to provide necessary information for regulators to determine adequate intakes. Liquid chromatography (LC) and mass spectrometry (MS) based methods for water-soluble vitamin analysis are abundant in the literature, but most focus on only fortified foods or dietary supplements or allow determination of only a single vitamin. In this work, a method based on LC/MS and LC/MS/MS has been developed to allow simultaneous quantitation of eight water-soluble vitamins, including multiple forms of vitamins B3 and B6, in a variety of fortified and unfortified food-matrix Standard Reference Materials (SRMs). Optimization of extraction of unbound vitamin forms and confirmation using data from external laboratories ensured accuracy in the assigned values, and addition of stable isotope labeled internal standards for each of the vitamins allowed for increased precision.
维生素对于改善和维持人体健康至关重要，而维生素的主要来源是饮食。测定常见食品原料中水溶性维生素的含量对于了解维生素摄入对人体健康的影响很重要，同时也为监管机构确定适宜摄入量提供必要信息。文献中基于液相色谱（LC）和质谱（MS）的水溶性维生素分析方法很多，但大多数仅关注强化食品或膳食补充剂，或者只能测定单一维生素。在本研究中，已开发出一种基于LC/MS和LC/MS/MS的方法，可同时定量多种强化和未强化食品基质标准参考物质（SRM）中的八种水溶性维生素，包括多种形式的维生素B3和B6。对未结合维生素形式的提取进行优化，并利用外部实验室的数据进行确认，确保了赋值的准确性，为每种维生素添加稳定同位素标记的内标提高了精密度。
