Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States.
Anal Chem. 2011 Jan 1;83(1):92-8. doi: 10.1021/ac101950r. Epub 2010 Nov 30.
Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have adequate intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography-mass spectrometry (LC-MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC-MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When possible, stable isotope labeled internal standards were employed for quantification.
维生素对人类健康至关重要，希望确保摄入足够这些重要营养素的个人通常会使用含有维生素的膳食补充剂。为了监测法规遵从性和研究特定人群的营养素摄入量，有必要对营养配方中的维生素进行测量。对于脂溶性和水溶性测量，液相色谱法（主要采用紫外吸收检测）已经非常成熟，但对于某些分析物存在局限性，并且在复杂基质中可能缺乏特异性。液相色谱-质谱法（LC-MS）为这些基质中的维生素测定提供了灵敏度和特异性，并且通常可以在单次分析中同时分析多种维生素。在这项工作中，开发了用于脂溶性和水溶性维生素的 LC-MS 方法，并将其应用于两种 NIST 标准参考物质中这些分析物的测量。在可能的情况下，采用稳定同位素标记的内标进行定量。
