Department of Chemistry, Rikkyo University, Toshima-ku, Tokyo 171-8501, Japan.
Magn Reson Chem. 2010 Mar;48(3):205-9. doi: 10.1002/mrc.2567.
We developed a new assignment technique of tryptophan residues using pulsed field gradient TOCSY-ROESY (PFG-TORO) and pulsed field gradient TOCSY-ROESY-TOCSY (PFG-TOROTO) techniques in water. Connectivity from betaH to zeta2H (H-7) via epsilon1H (H-1) and delta1H (H-2) in the TORO spectrum and from betaH to zeta3H (H-5) and eta2H (H-6) via epsilon1H (H-1) and delta1H (H-2) in the TOROTO spectrum could be able to assign each of the protons of the indole rings.
我们在水中使用脉冲场梯度 TOCSY-ROESY（PFG-TORO）和脉冲场梯度 TOCSY-ROESY-TOCSY（PFG-TOROTO）技术开发了一种新的色氨酸残基分配技术。在 TORO 光谱中，从 betaH 到 zeta2H（H-7）通过 epsilon1H（H-1）和 delta1H（H-2），以及在 TOROTO 光谱中从 betaH 到 zeta3H（H-5）和 eta2H（H-6）通过 epsilon1H（H-1）和 delta1H（H-2），可以分配吲哚环中每个质子的信号。
