人血清白蛋白在有并发症和无并发症眼部疾病中的氧化还原状态。

The redox state of human serum albumin in eye diseases with and without complications.

机构信息

Institute of Physiological Chemistry, Center for Physiological Medicine, Medical University of Graz, Austria.

出版信息

Acta Ophthalmol. 2011 Mar;89(2):e174-9. doi: 10.1111/j.1755-3768.2009.01824.x.

DOI:10.1111/j.1755-3768.2009.01824.x

PMID:20064117

Abstract

PURPOSE

To investigate the redox state of human serum albumin concerning cysteine-34 as a possible systemic redox marker in patients with different eye diseases with and without complications and with consideration of possible effects of age.

METHODS

Cataract (CAT), glaucoma, age-related macular degeneration (AMD), diabetes mellitus (DM), diabetic retinopathy and hypertension were the pathologies investigated. Albumin redox state concerning cysteine-34 was measured by high-performance liquid chromatography with fluorescence detection. The separation gives three fractions: the fully reduced form containing a thiol group, the disulphide form and a higher oxidized form. Statistical analysis was done by Student's t-test, analysis of variance and stepwise regression analysis.

RESULTS

Albumin as a systemic marker for oxidative stress was shifted to a more oxidized state by DM. An even stronger shift to the oxidized form was observed in patients with proliferative diabetic retinopathy. Notably, these effects were independent from age. In contrast, CAT and AMD had no influence on serum albumin redox state.

CONCLUSION

Serum albumin is not shifted to more oxidized forms by localized oxidative stress, but it is in systemic diseases like DM.

摘要

目的

研究人血清白蛋白的氧化还原状态与半胱氨酸 34 有关，作为一种可能的全身性氧化还原标志物，用于研究不同眼部疾病患者（伴有和不伴有并发症），并考虑年龄可能产生的影响。

方法

研究的病理包括白内障（CAT）、青光眼、年龄相关性黄斑变性（AMD）、糖尿病（DM）、糖尿病性视网膜病变和高血压。通过高效液相色谱法和荧光检测来测量与半胱氨酸 34 相关的白蛋白氧化还原状态。该分离给出了三种形式：含有巯基的完全还原形式、二硫化物形式和更高氧化形式。通过学生 t 检验、方差分析和逐步回归分析进行统计分析。