Division of Medicinal Chemistry, Otto Loewi Research Center, Medical University of Graz, Neue Stiftingtalstrasse 6, 8010 Graz, Austria.
Division of Endocrinology and Diabetology, Interdisciplinary Metabolic Medicine Trials Unit, Medical University of Graz, Auenbruggerplatz 15, 8036 Graz, Austria.
Int J Mol Sci. 2023 Nov 13;24(22):16256. doi: 10.3390/ijms242216256.
Oxidative stress is involved in the development, progression, and complications of diabetes mellitus (DM). Oxidative modification of human serum albumin's cysteine-34 is a marker for oxidative stress-related pathological conditions. We aimed to evaluate the redox state of albumin in patients with DM to investigate possible correlations with age, diabetes duration, and disease control status. Plasma aliquots were collected from 52 participants (26 type 1 and 26 type 2 DM). Patients were divided into two groups according to their glycated hemoglobin levels less than or equal to and greater than 58 mmol/L. Albumin redox state was assessed with high-performance liquid chromatography by fractionating it into human mercaptalbumin (HMA) and human nonmercaptalbumin 1 and 2 (HNA1 and HNA2). Albumin redox fractions were differently related to the age of study participants. In age-matched T1DM and T2DM groups, the albumin redox state was essentially the same. Irreversibly oxidized HNA2 was positively correlated with diabetes duration, especially in the T1DM group. HNA was increased in people with an increased HbA1c (>58 mmol/mol). Our results support the hypothesis that oxidative stress plays a crucial role in DM pathogenesis and emphasize the importance of diabetes control on systemic oxidative burden.
氧化应激参与糖尿病（DM）的发生、发展和并发症。人血清白蛋白半胱氨酸 34 的氧化修饰是氧化应激相关病理状态的标志物。我们旨在评估 DM 患者白蛋白的氧化还原状态，以研究其与年龄、糖尿病病程和疾病控制状态的可能相关性。从 52 名参与者（26 名 1 型和 26 名 2 型 DM）中采集血浆等分试样。根据糖化血红蛋白水平（HbA1c）≤58mmol/L 和＞58mmol/L 将患者分为两组。采用高效液相色谱法通过将白蛋白分离为人巯基白蛋白（HMA）和人非巯基白蛋白 1 和 2（HNA1 和 HNA2）来评估白蛋白的氧化还原状态。白蛋白氧化还原分数与研究参与者的年龄有不同的关系。在年龄匹配的 1 型和 2 型 DM 组中，白蛋白的氧化还原状态基本相同。不可逆氧化的 HNA2 与糖尿病病程呈正相关，尤其是在 1 型 DM 组中。HbA1c 升高的人群中 HNA 增加。我们的结果支持氧化应激在 DM 发病机制中起关键作用的假设，并强调控制糖尿病对全身氧化负荷的重要性。
