Inserm CIC3, Grenoble Clinical Research Center, Grenoble University Hospital, France.
Microvasc Res. 2010 Mar;79(2):102-8. doi: 10.1016/j.mvr.2010.01.001. Epub 2010 Jan 11.
The primary objective of this study was to evaluate 1-week reproducibility of post-occlusive reactive hyperemia (PORH) and local thermal hyperemia (LTH) assessed by single-point laser-Doppler flowmetry (LDF) on different skin sites. We also evaluated spatial reproducibility of both tests on the forearm. Finally, we assessed the influence of mental stress and room temperature variations on PORH and LTH.
We performed PORH and LTH assessing skin blood flow on the forearm and on the finger pad with LDF. We repeated the sequence 1 week later. We also performed PORH and LTH during mental stress (Stroop test) and at room temperatures of 21 degrees C and 27 degrees C. Data were expressed as cutaneous vascular conductance (CVC), as a function of baseline and as a function of 44 degrees C vasodilation (%CVC(44)). Reproducibility was expressed as within subject coefficients of variation (CV) and intra-class correlation coefficients (ICC).
Fourteen Caucasian healthy volunteers were recruited. Median age was 25 (2.7) and 50% were female. Median body mass index was 21.2 (5). PORH was reproducible on the finger, whether expressed as raw CVC (CV=25%; ICC=0.56) or as %CVC(44) (CV=24%; ICC=0.60). However, PORH showed poor reproducibility on the forearm. In the same way, LTH was reproducible on the finger pad when expressed as CVC (CV=17%; ICC=0.81) but not on the forearm. Spatial reproducibility was poor on the forearm. Elevated room temperature (27 degrees C) affected PORH and LTH on the finger pad (p<0.05) but not on the forearm.
Single-point LDF is a reproducible technique to assess PORH and LTH on the finger pad when data are expressed as raw CVC or %CVC(44). On the forearm, however, it shows great inter-day variability, probably due to spatial variability of capillary density. These results highlight the need for alternative techniques on the forearm.
本研究的主要目的是评估单点激光多普勒流量仪（LDF）评估的闭塞后反应性充血（PORH）和局部热充血（LTH）在不同皮肤部位的 1 周可重复性。我们还评估了前臂上这两种测试的空间可重复性。最后，我们评估了心理压力和室温变化对 PORH 和 LTH 的影响。
我们使用 LDF 在手背和手指垫上评估 PORH 和 LTH 对皮肤血流的影响。我们在 1 周后重复该序列。我们还在手背在 21°C 和 27°C 的室温下进行 PORH 和 LTH 测试。数据表示为皮肤血管传导率（CVC），表示为基础值和 44°C 血管扩张的函数（%CVC(44)）。可重复性表示为受试者内变异系数（CV）和组内相关系数（ICC）。
共纳入 14 名白种健康志愿者。中位年龄为 25（2.7）岁，50%为女性。中位体重指数为 21.2（5）。手指的 PORH 无论是以原始 CVC（CV=25%；ICC=0.56）还是以%CVC(44)（CV=24%；ICC=0.60）表示，都具有可重复性。然而，前臂的 PORH 可重复性差。同样，手指垫上的 LTH 以 CVC（CV=17%；ICC=0.81）表示时具有可重复性，但前臂上的 LTH 不具有可重复性。前臂的空间可重复性差。较高的室温（27°C）会影响手指垫上的 PORH 和 LTH（p<0.05），但不会影响前臂。
单点 LDF 是评估手指垫上 PORH 和 LTH 的一种可重复技术，当数据以原始 CVC 或%CVC(44)表示时。然而，在手背上，它显示出很大的日内变异性，可能是由于毛细血管密度的空间变异性所致。这些结果强调了在手臂上需要替代技术。
