Department of Public Health, Section of Environmental Health, University of Copenhagen, DK-1014 Copenhagen, Denmark.
Mutagenesis. 2010 Mar;25(2):109-11. doi: 10.1093/mutage/gep067. Epub 2010 Jan 11.
The alkaline single cell gel electrophoresis (comet) assay has become a widely used method for the detection of DNA damage and repair in cells and tissues. Still, it has been difficult to compare results from different investigators because of differences in assay conditions and because the data are reported in different units. The European Comet Assay Validation Group (ECVAG) was established for the purpose of validation of the comet assay with respect to measures of DNA damage formation and its repair. The results from this inter-laboratory validation trail showed a large variation in measured level of DNA damage and formamidopyrimidine DNA glycosylase-sensitive sites but the laboratories could detect concentration-dependent relationships in coded samples. Standardization of the results with reference standards decreased the inter-laboratory variation. The ECVAG trail indicates substantial reliability for the measurement of DNA damage by the comet assay but there is still a need for further validation to reduce both assay and inter-laboratory variation.
碱性单细胞凝胶电泳（彗星）试验已成为检测细胞和组织中 DNA 损伤和修复的广泛应用方法。然而，由于试验条件的差异以及数据以不同的单位报告，不同研究者之间的结果难以进行比较。建立欧洲彗星试验验证小组（ECVAG）的目的是验证彗星试验在 DNA 损伤形成及其修复方面的措施。这项实验室间验证试验的结果表明，测量的 DNA 损伤水平和甲酰胺嘧啶 DNA 糖基化酶敏感部位存在很大差异，但实验室可以检测到编码样本中浓度依赖性关系。用参比标准品对结果进行标准化降低了实验室间的变异性。ECVAG 试验表明，彗星试验测量 DNA 损伤具有很大的可靠性，但仍需要进一步验证，以减少试验和实验室间的变异性。
