Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
Spinal Cord. 2010 Jul;48(7):576-81. doi: 10.1038/sc.2009.170. Epub 2010 Jan 12.
Acellular spinal cord was prepared through chemical extraction, and its biocompatibility was studied.
Acellular scaffolds have been developed from various materials for tissue reconstruction, except for spinal cord. The objective of this study was to prepare acellular spinal cord and examine the biocompatibility of the scaffold.
This study was conducted at the Department of Orthopedics, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
The morphology of the acellular segments was revealed by scanning electron microscopy, immunohistochemistry, and hematoxylin and eosin stain. Biocompatibility was studied by immunohistochemistry.
Results show that in spinal cord scaffolds, cells, myelin sheath and axon of nerve fibers were eliminated, and three-dimensional supports of extracellular matrix were reserved. The component analytical results of the acellular spinal cord indicate that they contain laminin, fibronectin and collagen, which can facilitate and induce the regeneration of injured nerves, and enhance the adhesion and proliferation of cells. The acellular spinal cord has a three-dimensional structure and excellent biocompatibility.
Our data indicate that acellular spinal cord has certain biological properties and it may be a potential alternative scaffold for spinal cord tissue engineering.
通过化学提取制备去细胞脊髓，并研究其生物相容性。
除脊髓外，各种材料已被开发用于组织重建的去细胞支架。本研究旨在制备去细胞脊髓并研究支架的生物相容性。
本研究在第三军医大学新桥医院骨科进行。
通过扫描电子显微镜、免疫组织化学和苏木精-伊红染色观察去细胞段的形态。通过免疫组织化学研究生物相容性。
结果表明，在脊髓支架中，细胞、髓鞘和神经纤维轴突被消除，保留了细胞外基质的三维支架。去细胞脊髓的成分分析结果表明，它们含有层粘连蛋白、纤维连接蛋白和胶原蛋白，这有助于和诱导损伤神经的再生，并增强细胞的黏附和增殖。去细胞脊髓具有三维结构和良好的生物相容性。
我们的数据表明，去细胞脊髓具有一定的生物学特性，可能是脊髓组织工程的潜在替代支架。
