Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 1608582, Japan.
World J Gastroenterol. 2010 Jan 14;16(2):184-92. doi: 10.3748/wjg.v16.i2.184.
To elucidate the effect of antioxidants, resveratrol (RVT) and astaxanthin (AXN), on hepatitis C virus (HCV) replication.
We investigated the effect of recent popular antioxidant supplements on replication of the HCV replicon system OR6. RVT is a strong antioxidant and a kind of polyphenol that inhibits replication of various viruses. AXN is also a strong antioxidant. The replication of HCV RNA was assessed by the luciferase reporter assay. An additive effect of antioxidants on antiviral effects of interferon (IFN) and ribavirin (RBV) was investigated.
This is the first report to investigate the effect of RVT and AXN on HCV replication. In contrast to other reported viruses, RVT significantly enhanced HCV RNA replication. Vitamin E also enhanced HCV RNA replication as reported previously, although AXN did not affect replication. IFN and RBV significantly reduced HCV RNA replication, but these effects were dose-dependently hampered and attenuated by the addition of RVT. AXN did not affect antiviral effects of IFN or RBV.
These results suggested that RVT is not suitable as an antioxidant therapy for chronic hepatitis C.
阐明抗氧化剂白藜芦醇（RVT）和虾青素（AXN）对丙型肝炎病毒（HCV）复制的影响。
我们研究了最近流行的抗氧化补充剂对 HCV 复制子系统 OR6 的复制的影响。RVT 是一种强抗氧化剂和多酚，可抑制多种病毒的复制。AXN 也是一种强抗氧化剂。通过荧光素酶报告基因检测评估 HCV RNA 的复制。研究了抗氧化剂对干扰素（IFN）和利巴韦林（RBV）抗病毒作用的附加效应。
这是第一项研究 RVT 和 AXN 对 HCV 复制影响的报告。与其他报道的病毒不同，RVT 显著增强了 HCV RNA 的复制。正如先前报道的那样，维生素 E 也增强了 HCV RNA 的复制，尽管 AXN 对复制没有影响。IFN 和 RBV 显著降低了 HCV RNA 的复制，但这些作用被 RVT 的添加剂量依赖性地阻碍和减弱。AXN 对 IFN 或 RBV 的抗病毒作用没有影响。
这些结果表明，RVT 不适合作为慢性丙型肝炎的抗氧化剂治疗。
