[重组膜蛋白对绵羊多头绦虫幼虫的免疫保护作用]

[Immune protection of recombinant membrane protein against Taenia multiceps larvae in sheep].

作者信息

Zhang Zhuang-Zhi, Shi Bao-Xin, You Hong, Zhao Li, Tuerhong Yimiti, Wang Jin-Cheng, Zhang Wen-Bao, Cui De-Sheng, Guo Jin-Liang

机构信息

Institute of Veterinary Research, Xinjiang Academy of Animal Science, Urumqi, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Aug;27(4):336-9.

PMID:20066992

Abstract

OBJECTIVE

To investigate the immune protection of the recombinant membrane protein(45M) against larvae of Taenia multiceps in sheep.

METHODS

Twelve sheep were randomly divided into immune group and control group, and each sheep was immunized four times (three weeks interval) respectively with 50 microg recombinant 45M or GST emulsified with adjuvant. Serum samples were collected at pre-immunization and certain time after immunization, and were analyzed for IgG and IgM by ELISA. At the 105th day after the final inoculation, each sheep was orally challenged by 5000 eggs of T. multiceps. Two weeks after infection, the sheep were sacrificed, serum samples were collected, and number of cysts in brain tissue was counted. Activated oncospheres were cultured with 10% sera of immune group or control group, and observed by light microscopy.

RESULTS

The mean number of cysts was 1.5, and the average diameter of the cysts was 2.2 mm. Compared with control group, the reduction rate of cysts in immunized sheep was 68.9%. At the 72th hour after culture with serum of immunized sheep, about 90% oncospheres were killed by antiserum, and the oncospheres began to shrink or expand, and internal structure became fuzzy. ELISA showed that after final immunization (the 9th week), the levels of IgG in sera of immune group (2.32+/-0.76) were significantly higher than those of the control (0.70+/-0.42) (t=4.47, P<0.01). At the 24th week the levels of IgG and IgM in sera of immune group (1.53+/- 0.81, 0.90+/-0.26) were significantly higher than those of the control (0.64+/-0.43, 0.43+/-0.15) (P<0.01).

CONCLUSION

The recombinant 45M can induce certain humoral immune response.

摘要

目的

研究重组膜蛋白（45M）对绵羊多头绦虫幼虫的免疫保护作用。

方法

将12只绵羊随机分为免疫组和对照组，分别用50μg重组45M或与佐剂乳化的GST对每只绵羊进行4次免疫（间隔3周）。在免疫前和免疫后的特定时间采集血清样本，通过ELISA分析IgG和IgM。在最后一次接种后的第105天，每只绵羊口服5000枚多头绦虫虫卵进行攻击感染。感染2周后，处死绵羊，采集血清样本，计数脑组织中的包囊数量。用免疫组或对照组的10%血清培养激活的六钩蚴，并用光学显微镜观察。

结果

包囊平均数量为1.5个，包囊平均直径为2.2mm。与对照组相比，免疫绵羊的包囊减少率为68.9%。用免疫绵羊的血清培养72小时后，约90%的六钩蚴被抗血清杀死，六钩蚴开始萎缩或膨胀，内部结构变得模糊。ELISA结果显示，末次免疫后（第9周），免疫组血清中IgG水平（2.32±0.76）显著高于对照组（0.70±0.42）（t=4.47，P<0.01）。第24周时，免疫组血清中IgG和IgM水平（1.53±0.81，0.90±0.26）显著高于对照组（0.64±0.43，0.43±0.15）（P<0.01）。