[乌拉尔甘草中编码β-香树脂醇合酶的开放阅读框的克隆与鉴定]
[Cloning and characterization of open reading frame encoding beta-amyrin synthase in Glycyrrhiza uralensis].
作者信息
Shen Zhanyun, Liu Chunsheng, Wang Xueyong
机构信息
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(19):2438-40.
OBJECTIVE
To clone and sequence the open reading frame of beta-amyrin synthase (bAS) from Glycyrrhiza uralensis.
METHOD
The primers were designed according to the cDNA sequence of beta-amyrin synthase from G. glabra reported by Hiroaki HAYASHI, and the open reading frame of beta-amyrin synthase was cloned by RT-PCR strategy with the template of total RNA extracted from roots of G. uralensis.
RESULT
The GubAS (GenBank Accession number: FJ627179) was 2 289 bp in length encoding one pelypeptide of 762 amino acid. Deduced amino acid sequence had 99%, 92%, 90%, 90% and 89% homology to the amino acid sequence of G. glabra, Lotus japonicus, Pisum sativum, Medicago truncatula, Glycine max, respectively.
CONCLUSION
The open reading frame of bAS from G. uralensis is cloned and reported for the first time. The conclusion will provide a foundation for exploring the mechanism of triterpenes biosynthesis.
目的
克隆并测序乌拉尔甘草β-香树脂醇合酶（bAS）的开放阅读框。
方法
根据Hiroaki HAYASHI报道的光果甘草β-香树脂醇合酶的cDNA序列设计引物，以乌拉尔甘草根中提取的总RNA为模板，采用RT-PCR策略克隆β-香树脂醇合酶的开放阅读框。
结果
GubAS（GenBank登录号：FJ627179）长度为2289 bp，编码一个由762个氨基酸组成的多肽。推导的氨基酸序列与光果甘草、百脉根、豌豆、蒺藜苜蓿、大豆的氨基酸序列分别具有99%、92%、90%、90%和89%的同源性。
结论
首次克隆并报道了乌拉尔甘草bAS的开放阅读框。该结论将为探索三萜生物合成机制提供基础。
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