Zang Yi-mei, Li Yan-peng, Qiao Jing, Chen Hong-hao, Liu Chun-sheng
Yao Xue Xue Bao. 2015 Jul;50(7):906-9.
β-Amyrin synthase (β-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.
采用PCR-SSCP技术对来自6个不同地区的乌拉尔甘草β-香树脂醇合成酶（β-AS）基因进行分析并测序，进而确定β-AS基因单核苷酸多态性（SNP）与乌拉尔甘草产地之间的相关性。依据β-AS基因第1外显子94 bp位点处的1个编码单核苷酸多态性，可将乌拉尔甘草分为3种基因型。在这些基因型中，正品产地94A基因型的比例较高，与非正品产地的比例存在显著差异（P<0.001）。实验结果证明，不同产地94 bp位点处β-AS基因的不同基因型可能是导致乌拉尔甘草道地性的重要原因之一。
