Institut Supérieur de Biotechnologie de Sfax, Tunisie.
Connect Tissue Res. 2009;50(5):330-5.
The administration of antioxidants has been shown to enhance repair and healing processes in cutaneous tissue. Date seed oil (DSO) extract, which might be a potential source of natural antioxidants such as phenols and tocopherols, has been reported to be beneficial in the reduction of chemically induced oxidative stress in normal human skin. In this study, we investigated the protective effects of DSO against hydrogen peroxide (H(2)O(2))-induced oxidative stress in terms of lipid peroxidation, depletion of such endogenous antioxidant defense enzymes as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) using normal human epidermal melanocytes (NHEM). The results showed that DSO, endowed with a radical scavenging ability, decreased oxidative injury by inhibition of damage caused by H(2)O(2). Treatment of NHEM with DSO inhibited H(2)O(2)-induced lipid peroxidation. In addition, the extract inhibited H(2)O(2)-induced depletion of antioxidant defense components, such as SOD, CAT, and GPx. Our findings demonstrate that DSO is an efficient extract able to prevent melanocytes oxidative damage induced by H(2)O(2) exposure. Thus it may be a potential promising candidate, as a chemopreventive agent, in the development of melanocyte-related pathologies like vitiligo and melanoma.
抗氧化剂的应用已被证明可以促进皮肤组织的修复和愈合过程。芝麻籽油（DSO）提取物可能是酚类和生育酚等天然抗氧化剂的潜在来源，据报道，它有助于减少正常人体皮肤中化学诱导的氧化应激。在这项研究中，我们研究了 DSO 对过氧化氢（H2O2）诱导的氧化应激的保护作用，涉及脂质过氧化、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等内源性抗氧化防御酶的耗竭，使用正常人表皮黑素细胞（NHEM）。结果表明，DSO 具有自由基清除能力，通过抑制 H2O2 引起的损伤，减少氧化损伤。用 DSO 处理 NHEM 可抑制 H2O2 诱导的脂质过氧化。此外，该提取物抑制了 H2O2 诱导的抗氧化防御成分如 SOD、CAT 和 GPx 的耗竭。我们的研究结果表明，DSO 是一种有效的提取物，能够防止 H2O2 暴露引起的黑素细胞氧化损伤。因此，它可能是一种有前途的候选药物，作为化学预防剂，用于治疗与黑素细胞相关的疾病，如白癜风和黑色素瘤。
