Department of Oral and Maxillofacial Surgery, Saitama Medical University, Iruma-gun, Saitama, Japan.
FEBS Lett. 2010 Feb 19;584(4):817-24. doi: 10.1016/j.febslet.2010.01.001. Epub 2010 Jan 12.
Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts.
最近的研究表明，乙酰胆碱（ACh）在各种组织中发挥着重要作用，而 ACh 在骨代谢中的作用尚不清楚。在这里，我们证明 ACh 通过骨细胞中的烟碱型乙酰胆碱受体（nAChRs）和毒蕈碱型乙酰胆碱受体（mAChRs）诱导细胞增殖并降低碱性磷酸酶（ALP）活性。我们检测了几种 nAChRs 和 mAChRs 的 mRNA 表达。此外，我们还表明，在成骨细胞分化过程中，胆碱能成分上调，乙酰胆碱受体亚基/亚型改变。据我们所知，这是第一项表明成骨细胞表达特定的乙酰胆碱受体和胆碱能成分，并且 ACh 可能在调节成骨细胞增殖和分化中发挥作用的报告。
