Asdaq Syed Mohammed Basheeruddin, Inamdar Mohammed Naseeruddin, Asad Mohammed
Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore-560 034, India.
Pak J Pharm Sci. 2010 Jan;23(1):42-7.
The present study was undertaken to explore the interaction of garlic homogenate (GH) with propranolol (PRO) on ischemia-reperfusion injury (IRI) in isolated rat heart preparation. Albino rats were treated with GH at three different doses of 125 mg/kg, (GH-125), 250 mg/kg (GH-250) and 500 mg/kg (GH-500) for 30 days orally. The hearts were excised and mounted on modified Langendorff setup and subjected to 15 min global no flow ischemia and reperfused for 15 min. Pretreatment of animals with PRO, GH-125 and GH-250 (either alone or in combination) provided significant protection to myocardium from IRI damage as indicated by significant decrease in LDH and CK-MB activities in perfusate and an increase in activities of these enzymes in heart tissue homogenate. Similarly, the recovery (%) in developed tension and heart rate were significantly more in treated groups during post-ischemia when compared to control. Moreover, GH-250 either alone or with PRO showed significant increase in activities of antioxidant enzymes such as superoxide dismutase and catalase during IRI damage. However, GH-500 failed to show cardioprotective effect when given alone or along with PRO. These biochemical findings were supported by changes in histopathological studies.
本研究旨在探讨大蒜匀浆（GH）与普萘洛尔（PRO）对离体大鼠心脏缺血再灌注损伤（IRI）的相互作用。将白化大鼠分别以125 mg/kg（GH - 125）、250 mg/kg（GH - 250）和500 mg/kg（GH - 500）这三种不同剂量口服给予GH，持续30天。取出心脏并安装在改良的Langendorff装置上，进行15分钟的全心无血流缺血，然后再灌注15分钟。用PRO、GH - 125和GH - 250（单独或联合使用）预处理动物，可使心肌免受IRI损伤，这表现为灌注液中乳酸脱氢酶（LDH）和肌酸激酶同工酶（CK - MB）活性显著降低，以及心脏组织匀浆中这些酶的活性增加。同样，与对照组相比，治疗组在缺血后舒张期张力和心率的恢复率（%）显著更高。此外，GH - 250单独或与PRO联合使用时，在IRI损伤期间抗氧化酶如超氧化物歧化酶和过氧化氢酶的活性显著增加。然而，GH - 500单独或与PRO联合使用时均未显示出心脏保护作用。这些生化结果得到了组织病理学研究变化的支持。
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