Hesketh J E, Campbell G P, Whitelaw P F
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, U.K.
Biochem J. 1991 Mar 1;274 ( Pt 2)(Pt 2):607-9. doi: 10.1042/bj2740607.
3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, 130 mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in order to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta 2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.
将3T3成纤维细胞依次用25 mM - KCl/0.05%诺乃洗涤剂P40、130 mM - KCl/0.05%诺乃洗涤剂P40处理，最后用1%诺乃洗涤剂P40/1%脱氧胆酸盐处理，以便分别释放游离的、细胞骨架结合的和膜结合的多核糖体。膜结合部分富含膜蛋白β2 -微球蛋白的mRNA，而细胞骨架结合的多核糖体富含c - myc mRNA。肌动蛋白mRNA存在于游离的和细胞骨架结合的多核糖体中。结果表明，细胞骨架结合的多核糖体参与特定mRNA种类的翻译。
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