Hesketh J, Campbell G, Piechaczyk M, Blanchard J M
Division of Biochemical Sciences, Rowett Research Institute, Aberdeen, Scotland, U.K.
Biochem J. 1994 Feb 15;298 ( Pt 1)(Pt 1):143-8. doi: 10.1042/bj2980143.
The influence of the 3' untranslated region on mRNA localization was investigated by measuring the distribution of myc, beta-globin and hybrid myc-globin mRNAs between free, cytoskeletal-bound and membrane-bound polysomes in cells transfected with either control or chimeric gene constructs. c-myc sequences and beta-globin-coding sequences linked to the myc 3' untranslated region were present at greatest enrichment in cytoskeletal-bound polysomes. beta-Globin mRNA and myc-coding sequences linked to the beta-globin 3' untranslated region were recovered largely in the free polysomes. In situ hybridization confirmed that replacement of the c-myc 3' untranslated region by that of globin caused a relocalization of the mRNA. The results suggest that mRNA localization in differentiated eukaryotic cells depends on a mechanism that involves directional information in the 3' untranslated region of mRNAs.
通过测量在转染了对照或嵌合基因构建体的细胞中，游离、细胞骨架结合和膜结合多核糖体之间myc、β-珠蛋白以及杂交myc-珠蛋白mRNA的分布情况，研究了3'非翻译区对mRNA定位的影响。与myc 3'非翻译区相连的c-myc序列和β-珠蛋白编码序列在细胞骨架结合的多核糖体中富集程度最高。与β-珠蛋白3'非翻译区相连的β-珠蛋白mRNA和myc编码序列主要在游离多核糖体中回收。原位杂交证实，用珠蛋白的3'非翻译区替换c-myc的3'非翻译区会导致mRNA重新定位。结果表明，分化的真核细胞中的mRNA定位取决于一种涉及mRNA 3'非翻译区方向信息的机制。
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