Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.
J Econ Entomol. 2009 Dec;102(6):2310-5. doi: 10.1603/029.102.0637.
An understanding of the mechanisms of insecticide resistance in the bed bug, Cimex lectularius L., has the potential to lead to new approaches for the control of resistant populations. We used the cytochrome P450 monooxygenase (P450) inhibitor piperonyl butoxide (PBO) to assess the role of P450s in deltamethrin resistance in three field-collected bed bug strains, LA-1, CIN-1 and WOR-1. In addition, we exposed two highly resistant strains, CIN-1 and WOR-1 (resistance ratio [RR] >2,500-fold), to dry residues of piperonyl butoxide-synergized pyrethroid formulations to determine the utility of synergism by PBO. Piperonyl butoxide synergized deltamethrin in all three strains, but its impact was variable. The synergistic ratio varied from 40 in CIN-1 to 176 in WOR-1. Because the resistance ratio for each strain after piperonyl butoxide treatment was 174 and 39, respectively, our results suggest that P450s have some involvement in deltamethrin resistance, but other resistance mechanisms must be involved as well. No significant synergistic effect of formulated deltamethrin was observed with the addition of synergized pyrethrins or formulated piperonyl butoxide in the CIN-1 strain, but synergism occurred in the WOR-1 strain. Addition of PBO to pyrethroids is not a comprehensive solution to pyrethroid resistance because strains vary in both overall resistance level and the proportion of that resistance attributable to P450s.
了解臭虫（Cimex lectularius L.）对杀虫剂的抗药性机制，有可能为控制具有抗药性的臭虫种群提供新的方法。我们使用细胞色素 P450 单加氧酶（P450）抑制剂增效醚（PBO）来评估 P450 在三种野外采集的臭虫品系 LA-1、CIN-1 和 WOR-1 对溴氰菊酯抗性中的作用。此外，我们还将两种高度抗药性的品系 CIN-1 和 WOR-1（抗性比 [RR] >2500 倍）暴露于增效醚协同增效的拟除虫菊酯干粉残效剂中，以确定增效醚协同作用的效用。增效醚协同溴氰菊酯在所有三种品系中都有效，但效果不同。协同比从 CIN-1 的 40 到 WOR-1 的 176 不等。由于经增效醚处理后每种品系的抗性比分别为 174 和 39，我们的结果表明 P450 参与了溴氰菊酯的抗性，但也必须涉及其他抗性机制。在 CIN-1 品系中，加入协同增效的拟除虫菊酯或增效醚配制品对溴氰菊酯的协同作用不明显，但在 WOR-1 品系中却有协同作用。由于在 CIN-1 品系中，加入增效醚或协同增效的拟除虫菊酯对拟除虫菊酯的协同作用不明显，但在 WOR-1 品系中却有协同作用。由于在 CIN-1 品系中，加入增效醚或协同增效的拟除虫菊酯对拟除虫菊酯的协同作用不明显，但在 WOR-1 品系中却有协同作用。在加入增效醚后，加入增效醚并不能完全解决拟除虫菊酯的抗药性问题，因为不同品系的总体抗药性水平和归因于 P450 的抗药性比例不同。
