Teng Songshan, Liu Yanping, Zhao Lei
College of Life Sciences, Shandong Normal University, Jinan 250014, China.
Wei Sheng Wu Xue Bao. 2010 Nov;50(11):1503-9.
We Isolated and characterized 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing endophytic bacteria from halophyte Suaeda salsa to understand the interactions between endophytes and halophyte.
ACC deaminase-containing endophytic bacteria were isolated from root, stalk and leaf of Suaeda salsa and were identified based on morphological, physiological-biochemical properties, API and 16S rRNA sequence analysis. Isolates were evaluated for their ACC deaminase, antifungal, protease activity, siderophores and phytohormones, such as indole-3-acetic acid, gibberellic acid and abscisic acid production, as well as atmospheric nitrogen fixation and phosphate solubilization.
Four ACC deaminase-containing endophytic bacteria strains named as LP11, SS12, TW1 and TW2 were isolated and identified as Pseudomonas oryzihabitans, Pseudomonas sp., Pantoea agglomerans and Pseudomonas putida respectively. All the strains possessed the phosphate-solubilizing ability and could produce siderophores and phytohormones more or less. None of them could fix atmospheric nitrogen or produce protease. Only strain SS12 showed antagonism against two phytopathogenic fungi viz Fusarium oxysporum f. sp. conglutinans and F. oxysporum f. sp. cucumerinum.
ACC deaminase-containing endophytic bacteria of Pseudomonas sp. and Pantoea sp. isolated from halophyte Suaeda salsa have abundant biological characteristics related to plant growth promotion, stress homeostasis regulation and biocontrol activity.
从盐生植物盐地碱蓬中分离并鉴定含1-氨基环丙烷-1-羧酸（ACC）脱氨酶的内生细菌，以了解内生菌与盐生植物之间的相互作用。
从盐地碱蓬的根、茎和叶中分离含ACC脱氨酶的内生细菌，并根据形态学、生理生化特性、API和16S rRNA序列分析进行鉴定。对分离菌株进行ACC脱氨酶、抗真菌、蛋白酶活性、铁载体以及植物激素（如吲哚-3-乙酸、赤霉素和脱落酸）产生情况的评估，同时评估其大气固氮和溶磷能力。
分离出四株含ACC脱氨酶的内生细菌菌株，分别命名为LP11、SS12、TW1和TW2，经鉴定分别为栖稻假单胞菌、假单胞菌属、成团泛菌和恶臭假单胞菌。所有菌株均具有溶磷能力，且或多或少能产生铁载体和植物激素。它们均不能固定大气氮或产生蛋白酶。只有菌株SS12对两种植物病原真菌即尖孢镰刀菌黄瓜专化型和尖孢镰刀菌菜豆专化型表现出拮抗作用。
从盐生植物盐地碱蓬中分离出的假单胞菌属和泛菌属含ACC脱氨酶的内生细菌具有丰富的与促进植物生长、调节胁迫稳态和生物防治活性相关的生物学特性。
