Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.
Tissue Eng Part C Methods. 2010 Oct;16(5):1051-8. doi: 10.1089/ten.TEC.2009.0071.
This study was performed to assess the bone healing supporting characteristics of porous calcium phosphate (Ca-P) cement when implanted in a rabbit segmental defect model as well as to determine the reliability of torque testing as a method to verify bone healing. The middiaphyseal radius was chosen as the area to create bilaterally increasing defect sizes (5, 10, and 15 mm), which were either filled with porous Ca-P cement or left open as a control. After 12 weeks of implantation, torque test measurements as well as histological and radiographic evaluation were performed. In two of the open 15 mm control defects, bone bridging was visible at the radiographic and histological evaluation. Bone was observed to be present in all porous Ca-P cement implants (5, 10, and 15 mm defects) after 12 weeks. No significant differences in torque measurements were observed between the 5 and 10 mm filled and open control defects using a t-test. In addition, the mechanical strength of all operated specimens was similar compared with nonoperated bone samples. The torsion data for the 15 mm open defect appeared to be lower compared with the filled 15 mm defect, but no significant difference could be proven. Within the limitation of the study design, porous Ca-P cement implants demonstrated osteoconductive properties and confirmed to be a suitable scaffold material in a weight-bearing situation. Further, the used torque testing method was found to be unreliable for testing the mechanical properties of the healed bone defect.
本研究旨在评估多孔磷酸钙（Ca-P）水泥在兔节段性缺损模型中作为骨愈合支持物的特性，并确定扭矩测试作为验证骨愈合的方法的可靠性。选择桡骨中段作为区域，创建双侧逐渐增大的缺损大小（5、10 和 15mm），这些缺损要么用多孔 Ca-P 水泥填充，要么作为对照留空。植入 12 周后，进行扭矩测试测量以及组织学和影像学评估。在两个 15mm 对照缺损中，影像学和组织学评估可见骨桥形成。在 12 周后，所有多孔 Ca-P 水泥植入物（5、10 和 15mm 缺损）中均观察到骨存在。使用 t 检验，在填充和开放对照缺损的 5 和 10mm 之间未观察到扭矩测量值有显著差异。此外，与非手术骨样本相比，所有手术标本的机械强度相似。与填充的 15mm 缺损相比，15mm 开放缺损的扭转数据似乎较低，但无法证明存在显著差异。在研究设计的限制范围内，多孔 Ca-P 水泥植入物表现出骨诱导特性，并被证实是承重情况下合适的支架材料。此外，所使用的扭矩测试方法被发现不适用于测试愈合骨缺损的机械性能。
