人 RNA 聚合酶 I 驱动的犬细胞流感病毒反向遗传学。
Human RNA polymerase I-driven reverse genetics for influenza a virus in canine cells.
机构信息
Novartis Vaccines and Diagnostics, 45 Sidney Street, Cambridge, MA 02139, USA.
出版信息
J Virol. 2010 Apr;84(7):3721-5. doi: 10.1128/JVI.01925-09. Epub 2010 Jan 13.
Abstract
We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a nonendogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.
摘要
我们在犬肾细胞系 Madin-Darby 犬肾细胞 33016-PF 中建立了一个受人类 RNA 聚合酶 I（pol I）驱动的流感病毒反向遗传学（RG）系统，该系统已获得流感疫苗生产许可。RNA pol I 聚合酶通常仅在与其起源物种密切相关的细胞中具有活性。然而，我们表明，一个非内源性 RNA pol I 启动子可以在犬细胞系中有效地拯救流感 A 病毒。该系统的应用允许高效生成病毒株，并为流感疫苗生产提供了一种替代方法。
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