通过酶联免疫吸附测定法检测春季角结膜炎患者泪膜免疫球蛋白E（IgE）水平。

Tearfilm immunoglobulin E (IgE) level in vernal keratoconjunctivitis by ELISA.

作者信息

Pokharel S, Shah D N, Joshi S N, Choudhary M

机构信息

Ophthalmology Department, Kathmandu Medical College, Sinamangal, Nepal.

出版信息

Kathmandu Univ Med J (KUMJ). 2009 Apr-Jun;7(26):104-8. doi: 10.3126/kumj.v7i2.2700.

DOI:10.3126/kumj.v7i2.2700

PMID:20071840

Abstract

BACKGROUND

Vernal keratoconjunctivitis (VKC) is recurrent chronic allergic conjunctivitis occurring in the prepubertal age-group with secondary involvement of the cornea and is self-limiting in character. The disease is prevalent worldwide but it shows predominance in the areas with dry and warm climate including the South Asia. VKC represents about 3% of the serious ophthalmic disease in some parts of the world where the prevalence is rate is high.Type I hypersensitivity reaction which is IgE-dependent and type IV hypersensitivity reaction have been implicated for the pathogenesis VKC.

OBJECTIVE

To determine level of immunoglobulin E (IgE) in the tear fi lm of patients with Vernal Keratoconjunctivitis (VKC) attending outpatient department of BP Koirala Lions Centre for Ophthalmic Studies (BPKLCOS).

MATERIALS AND METHODS

Thirty-four VKC patients and thirty-four controls were included in this study. Tear samples were collected using micro- capillary glass tube method and tear IgE levels were measured using an enzyme - linked immunosorbent assay (ELISA).

RESULTS

There was high concentration of tear IgE level in VKC (95.09IU/ml) than in controls (1.63IU/ml) though the difference was not statistically significant (p=0.16). No statistically significant difference was observed in male and female gender within VKC group and when compared with control group (in male group, p=0.21 and in female group, p=0.26). There was no statistically significant difference observed in tear IgE level in different age groups within VKC group and when compared with control group (p=0.30). The result did not show any significant difference in tear IgE level with respect to the duration of the disease (p=0.23).There was no statistically significant difference in tear IgE level with different episodes of VKC (p=0.69). No statistically significant difference of IgE concentration in tear was seen among different types of VKC (p=0.53) and grades of tarsal and limbal papillae (p=0.72).

CONCLUSION

There was high concentration of tear IgE level observed in VKC.

摘要

背景

春季角结膜炎（VKC）是一种复发性慢性过敏性结膜炎，发生于青春期前年龄组，角膜继发受累，具有自限性。该疾病在全球范围内普遍存在，但在包括南亚在内的干燥温暖气候地区更为常见。在世界上一些患病率较高的地区，VKC约占严重眼科疾病的3%。I型超敏反应（IgE依赖型）和IV型超敏反应与VKC的发病机制有关。

目的

测定在BP Koirala狮子眼科研究中心门诊部就诊的春季角结膜炎（VKC）患者泪膜中免疫球蛋白E（IgE）的水平。

材料与方法

本研究纳入34例VKC患者和34例对照。采用微量毛细管玻璃管法收集泪液样本，并使用酶联免疫吸附测定（ELISA）法测量泪液IgE水平。

结果

VKC患者泪液IgE水平（95.09IU/ml）高于对照组（1.63IU/ml），尽管差异无统计学意义（p = 0.16）。VKC组内男性和女性之间以及与对照组相比，未观察到统计学上的显著差异（男性组，p = 0.21；女性组，p = 0.26）。VKC组内不同年龄组之间以及与对照组相比，泪液IgE水平未观察到统计学上的显著差异（p = 0.30）。结果显示泪液IgE水平在疾病持续时间方面无显著差异（p = 0.23）。VKC不同发作次数的泪液IgE水平无统计学显著差异（p = 0.69）。不同类型的VKC之间以及睑板和角膜缘乳头分级之间，泪液中IgE浓度无统计学显著差异（p = 0.53和p = 0.72）。