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利用快速扫描光度计对生物材料中的多组分系统进行检测。

The examination of multicomponent systems in biological materials by means of a rapid scanning photometer.

作者信息

Lübbers D W, Wodick R

出版信息

Appl Opt. 1969 May 1;8(5):1055-62. doi: 10.1364/AO.8.001055.

Abstract

Only photometers scanning a large range of wavelengths provide the amount of information which is necessary to analyze multicomponent systems in a general form. After a description of a rapid scanning photometer and a discussion of the recording problems, the mathematical method is described which allows an optimal analysis of the concentrations of the single components of a multicomponent system. The error involved in this procedure is demonstrated by the calculation of the four nucleotides in a mixture. The long range photometry has been successfully applied to other problems such as measurements by reflected light and analyses of inhomogeneously distributed substances in living tissues.

摘要

只有能够扫描大范围波长的光度计才能提供以一般形式分析多组分系统所需的信息量。在描述了一种快速扫描光度计并讨论了记录问题之后,介绍了一种数学方法,该方法能够对多组分系统中各单一成分的浓度进行优化分析。通过计算混合物中的四种核苷酸,证明了该过程中涉及的误差。长程光度法已成功应用于其他问题,如反射光测量和生物组织中不均匀分布物质的分析。

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