Vaupel P W, Otte J, Manz R
Radiat Environ Biophys. 1982;20(4):289-300. doi: 10.1007/BF01323754.
The oxyhemoglobin saturation (HbO2) of single red blood cells within tumor microvessels (diameter: 3-12 micrometers) of DS-Carcinosarcoma was studied using a cryophotometric micromethod. In untreated control tumors (mean tissue temperature approx. 35 degrees C) the measured values scattered over the whole saturation range from zero to 100 sat. %, the mean being 51 sat. %. Upon heating at 40 degrees C for 30 min, the oxygenation of the tumor tissue significantly improved as compared with control conditions. After 40 degrees C-hyperthermia a mean oxyhemoglobin saturation of 66 sat. % was obtained. In contradistinction to this, after 43 degrees C-hyperthermia the tumor oxygenation was significantly lower and reached a mean HbO2 saturation value of 47 sat. %. A further temperature rise to 45 degrees C caused the oxygenation to drop drastically (mean oxyhemoglobin saturation value: 24 sat. %). This is due to a severe restriction of nutritive blood flow. The changes in tumor oxygenation after hyperthermia seem to be predominantly mediated through changes in tumor blood flow, including tumor microcirculation, which showed a similar temperature dependence. Metabolic effects probably play a minor role in the oxyhemoglobin saturation distribution within tumor microvessels.
采用低温光度显微法研究了DS-癌肉瘤肿瘤微血管（直径：3 - 12微米）内单个红细胞的氧合血红蛋白饱和度（HbO2）。在未经治疗的对照肿瘤（平均组织温度约35℃）中，测量值在从零到100饱和%的整个饱和度范围内分散，平均值为51饱和%。在40℃加热30分钟后，与对照条件相比，肿瘤组织的氧合显著改善。40℃热疗后，平均氧合血红蛋白饱和度为66饱和%。与此相反，43℃热疗后肿瘤氧合显著降低，平均HbO2饱和度值达到47饱和%。进一步升温至45℃导致氧合急剧下降（平均氧合血红蛋白饱和度值：24饱和%）。这是由于营养性血流的严重受限。热疗后肿瘤氧合的变化似乎主要通过肿瘤血流的变化介导，包括肿瘤微循环，其显示出类似的温度依赖性。代谢效应可能在肿瘤微血管内氧合血红蛋白饱和度分布中起次要作用。
