Hughes Jeffrey, Yadava Preeti, Mesaros Ryan
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, USA.
Methods Mol Biol. 2010;605:445-59. doi: 10.1007/978-1-60327-360-2_31.
With the recent discovery of small interfering RNA (siRNA), to silence the expression of genes in vitro and in vivo, there has been a need to deliver these molecules to the cell nucleus. Forming a lipid/nucleic acid complex has become a solution and is explored here. Certain methods and ideas are used, such as: the positive/negative electrostatic interaction with a cationic lipid and an anionic RNA molecule, the size of the lipid vesicle aiding the uptake target tissues, targeted lipoplexes which can increase efficiency, and the protection of the siRNA molecule from the natural defenses of the immune system. Many lipid formulations exist and can be experimented with to achieve varying results depending on the application.
随着近期小干扰RNA（siRNA）的发现，为了在体外和体内使基因表达沉默，就需要将这些分子递送至细胞核。形成脂质/核酸复合物已成为一种解决方案，本文对此进行探讨。采用了某些方法和理念，例如：阳离子脂质与阴离子RNA分子之间的正负静电相互作用、有助于摄取靶组织的脂质囊泡大小、可提高效率的靶向脂质体，以及保护siRNA分子免受免疫系统天然防御机制的影响。存在许多脂质制剂，可根据应用进行试验以获得不同结果。
