Department of Dermatology, Catholic University of the Sacred Heart, Rome, Italy.
Int J Immunopathol Pharmacol. 2009 Oct-Dec;22(4):1067-75. doi: 10.1177/039463200902200423.
Psoriasis is a multifactorial skin dermatosis characterized in its classical form by erythematous and hyperkeratotic plaques on extensor surfaces of the body, that in most cases can be managed therapeutically by topical agents. Hyperproliferation and a marked inflammation in both epidermis and dermis are thought to be driven by interaction of activated type-1 T lymphocytes and antigen-presenting cells and keratinocytes that release several proinflammatory and immunomodulating molecules. The aim of this study is to investigate whether tetrabromofluorecin, commonly know as eosin, a classical compound traditionally topically used in psoriasis for its presumed anti-inflammatory activities, is able to modulate the production of TNF-alpha, IL-6 and IL-8 that are recognized as the most active and characterized cytokines in the pathogenesis of this skin disorder. HaCaT cell line was used to verify the effects on epidermal inflammation by eosin at scalar doses after testing the viability of cells. Two different population of cells, one stimulated by IFNgamma and one non-stimulated, were cultivated in presence of tolerable concentrations. The expression and release of IL-6, IL-8, IL-10, and TNF-alpha were analysed by RT-PCR and ELISA, respectively. Our results show that tolerable concentrations of eosin were 0.05%, 0.02%, and 0.01%. The expression and production of TNFalpha, IL-8 and IL-6 were dramatically reduced in presence of eosin 0.05% and 0.02% and the action of eosin was more pronounced on TNF-alpha. In agreement with clinical data, our results show that in presence of tolerable concentrations, eosin seems to influence remarkably the production of three important cytokines involved in the hyperproliferation and inflammatory process, giving a specific explanation of its efficacy and supporting its topical use in the clinical setting.
银屑病是一种多因素的皮肤疾病，其典型形式表现为身体伸展表面的红斑和角化过度斑块，在大多数情况下，可通过局部治疗药物进行治疗。表皮和真皮的过度增生和明显炎症被认为是由激活的 1 型 T 淋巴细胞和抗原呈递细胞与角质形成细胞相互作用驱动的，后者释放几种促炎和免疫调节分子。本研究旨在探讨四溴荧光素（通常称为曙红）是否能够调节 TNF-α、IL-6 和 IL-8 的产生，曙红是一种传统上用于银屑病的经典化合物，因其假定的抗炎活性而被广泛应用。这些细胞因子被认为是这种皮肤疾病发病机制中最活跃和最具特征的细胞因子。HaCaT 细胞系被用于验证曙红在测试细胞活力后在标量剂量下对表皮炎症的影响。在可耐受浓度下，培养了两种不同的细胞群，一种用 IFNγ 刺激，另一种未刺激。通过 RT-PCR 和 ELISA 分别分析 IL-6、IL-8、IL-10 和 TNF-α 的表达和释放。我们的结果表明，曙红的可耐受浓度为 0.05%、0.02%和 0.01%。在 0.05%和 0.02%曙红存在的情况下，TNF-α、IL-8 和 IL-6 的表达和产生显著降低，曙红的作用在 TNF-α 上更为明显。与临床数据一致，我们的结果表明，在可耐受浓度下，曙红似乎显著影响三种重要细胞因子的产生，这些细胞因子参与了过度增殖和炎症过程，为其疗效提供了具体的解释，并支持其在临床环境中的局部应用。
