Department of Neurology and MIND Institute, University of California at Davis, Sacramento, Calif 95817, USA.
Stroke. 2010 Mar;41(3):538-43. doi: 10.1161/STROKEAHA.109.572537. Epub 2010 Jan 14.
This study investigated the effects of intravenous recombinant Fv-Hsp70 protein on infarction volume and behavior after experimental ischemic stroke.
Focal cerebral ischemia was produced by occluding the middle cerebral artery using the intraluminal suture technique. Rats subjected to 2 hours of focal ischemia were allowed to survive 24 hours. At 2(1/4) hours and 3 hours after onset of ischemia, Fv-Hsp70 recombinant protein (0.5 mg/kg) or saline was injected through the tail vein. Sensorimotor function and infarction volume were assessed at 24 hours after ischemia.
Administration of Fv-Hsp70 after focal cerebral ischemia significantly decreased infarct volume by 68% and significantly improved sensorimotor function compared with the saline-treated control group. Western blots showed Fv-Hsp70 in ischemic but not in control brain; and Fv-Hsp70 suppressed endogenous Hsp70.
Fv-Hsp70 protected the ischemic brain in this experimental stroke model.
本研究旨在探讨静脉内重组 Fv-Hsp70 蛋白对实验性缺血性卒中后梗死体积和行为的影响。
采用血管内缝线技术阻塞大脑中动脉产生局灶性脑缺血。对经历 2 小时局灶性缺血的大鼠,允许其存活 24 小时。在缺血发作后 2(1/4)小时和 3 小时,通过尾静脉注射 Fv-Hsp70 重组蛋白(0.5mg/kg)或生理盐水。在缺血后 24 小时评估感觉运动功能和梗死体积。
与生理盐水处理的对照组相比,局灶性脑缺血后给予 Fv-Hsp70 治疗显著降低了 68%的梗死体积,并显著改善了感觉运动功能。Western blot 显示 Fv-Hsp70 存在于缺血性脑但不存在于对照组脑;Fv-Hsp70 抑制了内源性 Hsp70。
Fv-Hsp70 对该实验性卒中模型中的缺血性脑具有保护作用。