Kushnir Mark M, Rockwood Alan L, Yue Bingfang, Meikle A Wayne
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.
Methods Mol Biol. 2010;603:219-28. doi: 10.1007/978-1-60761-459-3_20.
Measurement of low concentrations of estrogens, encountered in pre-pubertal children, men, and postmenopausal women, is important for numerous clinical applications. We describe a method for high sensitivity analysis of estrogens that uses two-dimensional chromatographic separation and tandem mass spectrometry detection. Aliquots of serum or plasma samples are combined with stable isotope-labeled internal standard and estrogens are extracted with methyl t-butyl ether. The solvent is evaporated, estrogens derivatized to form dansyl derivatives, and the samples are analyzed. Quantitation is performed using triple quadrupole mass spectrometer equipped with electrospray ion source using positive ion mode ionization and multiple reaction monitoring acquisition.
测量青春期前儿童、男性和绝经后女性体内低浓度雌激素，对众多临床应用而言至关重要。我们描述了一种雌激素高灵敏度分析方法，该方法采用二维色谱分离和串联质谱检测。将血清或血浆样本的等分试样与稳定同位素标记的内标混合，并用甲基叔丁基醚萃取雌激素。蒸发溶剂，将雌激素衍生化形成丹磺酰衍生物，然后对样品进行分析。使用配备电喷雾离子源的三重四极杆质谱仪，采用正离子模式电离和多反应监测采集进行定量分析。
