Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, Route de Saint-Cyr; Versailles Cedex, France.
Dev Dyn. 2010 Mar;239(3):747-62. doi: 10.1002/dvdy.22184.
The zebrafish is a well established model system for studying neural development, yet neurulation remains poorly understood in this organism. In particular, the morphogenetic movements that shape the posterior neural tube (PNT) have not been described. Using tools for imaging neural tissue and tracking the behavior of cells in real time, we provide the first comprehensive analysis of the cellular events shaping the PNT. We observe that this tissue is formed in a stepwise manner, beginning with merging of presumptive neural domains in the tailbud (Stage 1); followed by neural convergence and infolding to shape the neural rod (Stage 2); and continued elongation of the PNT, in absence of further convergence (Stage 3). We further demonstrate that cell proliferation plays only a minimal role in PNT elongation. Overall, these mechanisms resemble those previously described in anterior regions, suggesting that, in contrast to amniotes, neurulation is a fairly uniform process in zebrafish.
斑马鱼是研究神经发育的成熟模式生物，但在该生物中神经胚形成仍知之甚少。特别是，尚未描述塑造后脑神经管（PNT）的形态发生运动。我们使用用于成像神经组织和实时跟踪细胞行为的工具，提供了对塑造 PNT 的细胞事件的首次全面分析。我们观察到，该组织是逐步形成的，首先是在尾芽中假定的神经区域融合（阶段 1）；接着是神经收敛和内陷以形成神经杆（阶段 2）；以及在没有进一步收敛的情况下继续延长 PNT（阶段 3）。我们进一步证明细胞增殖在 PNT 伸长中仅起很小的作用。总体而言，这些机制类似于先前在前区域描述的机制，表明与羊膜动物相比，神经胚形成在斑马鱼中是一个相当均匀的过程。
