Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Elife. 2020 Mar 31;9:e48964. doi: 10.7554/eLife.48964.
An extracellular matrix of Fibronectin adheres the neural tube to the two flanking columns of paraxial mesoderm and is required for normal vertebrate development. Here, we find that the bilaterally symmetric interfaces between the zebrafish neural tube and paraxial mesoderm function as optimally engineered adhesive lap joints with rounded edges, graded Fibronectin 'adhesive' and an arced adhesive spew filet. Fibronectin is a 'smart adhesive' that remodels to the lateral edges of the neural tube-paraxial mesoderm interfaces where shear stress is highest. Fibronectin remodeling is mechanically responsive to contralateral variation morphogenesis, and Fibronectin-mediated inter-tissue adhesion is required for bilaterally symmetric morphogenesis of the paraxial mesoderm. Strikingly, however, perturbation of the Fibronectin matrix rescues the neural tube convergence defect of mutants. Therefore, Fibronectin-mediated inter-tissue adhesion dynamically coordinates bilaterally symmetric morphogenesis of the vertebrate trunk but predisposes the neural tube to convergence defects that lead to spina bifida.
纤连蛋白的细胞外基质将神经管附着到两侧的轴旁中胚层柱上，这对于正常的脊椎动物发育是必需的。在这里，我们发现斑马鱼神经管和轴旁中胚层之间的双侧对称界面作为优化设计的具有圆形边缘的黏合搭接接头，具有分级的纤连蛋白“黏附剂”和弧形黏附剂喷丝。纤连蛋白是一种“智能黏附剂”，可重塑为神经管-轴旁中胚层界面的侧向边缘，此处剪切力最高。纤连蛋白的重塑对相反侧的形态发生变化具有机械响应，并且纤连蛋白介导的组织间黏附对于轴旁中胚层的双侧对称形态发生是必需的。然而，令人惊讶的是，纤连蛋白基质的扰动挽救了 突变体的神经管汇聚缺陷。因此，纤连蛋白介导的组织间黏附动态协调脊椎动物躯干的双侧对称形态发生，但易使神经管发生会聚缺陷，导致脊柱裂。
