Bruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany.
Chemphyschem. 2010 Feb 22;11(3):689-95. doi: 10.1002/cphc.200900772.
Provided that (13)C-detected NMR experiments are either preferable or complementary to (1)H detection, we report here tools to determine C(alpha)-C', C'-N, and C(alpha)-H(alpha) residual dipolar couplings on the basis of the CON experiment. The coupling constants determined on ubiquitin are consistent with the subset measured with the (1)H-detected HNCO sequences. Since the utilization of residual dipolar couplings may depend on the mobility of the involved nuclei, we also provide tools to measure longitudinal and transverse relaxation rates of N and C'. This new set of experiments is a further development of a whole strategy based on (13)C direct-detection NMR spectroscopy for the study of biological macromolecules.
只要 (13)C 检测 NMR 实验优于或补充 (1)H 检测，我们就在这里报告工具，以便根据 CON 实验确定 C(alpha)-C'、C'-N 和 C(alpha)-H(alpha)的残余偶极耦合。在泛素上确定的耦合常数与用 (1)H 检测 HNCO 序列测量的子集一致。由于残余偶极耦合的利用可能取决于涉及核的流动性，我们还提供了用于测量 N 和 C'的纵向和横向弛豫率的工具。这组新的实验是基于 (13)C 直接检测 NMR 光谱学研究生物大分子的整体策略的进一步发展。
