Colombo P, Di Blasi F, Magrin S, Fabiano C, Di Marco V, D'Amelio L, Lojacono F, Spinelli G, Craxì A
Dipartimento di Biologia Cellulare e dello Sviluppo, University of Palermo, Italy.
J Hepatol. 1991 Jan;12(1):64-9. doi: 10.1016/0168-8278(91)90911-t.
Hepatitis B virus deoxyribonucleic acid (HBV-DNA) was studied by Southern blot analysis in liver biopsy specimens from 75 HBsAg-positive patients with chronic liver disease living in southern Italy. Twenty-seven of the patients were hepatitis delta virus (HDV) superinfected. Intrahepatic HBV-DNA was detected in 54 (72%) patients, 32 (59%) of them with replicative forms. The presence of replicative forms was directly related to liver HBcAg and inversely related to liver HDAg, as shown by multivariate analysis. However, 14 patients with intrahepatic HBV-DNA non-replicative pattern and about half of HDV-infected patients were liver HBcAg and/or serum HBV-DNA positive, mostly in low amounts. Histological inflammatory activity was strongly related to liver HBcAg expression regardless of HDV superinfection, as confirmed by multivariate analysis. Our results confirm previous studies about the concordance between intrahepatic HBV-DNA replicative pattern and liver HBcAg expression and about inhibition by HDV of high-level HBV replication. However, they suggest that low-level HBV replication may have an important role in causing liver damage also among HDV-infected patients, in a population where the spreading of HBV and HDV is a naturally occurring event.
通过Southern印迹分析，对居住在意大利南部的75例慢性肝病HBsAg阳性患者的肝活检标本进行了乙型肝炎病毒脱氧核糖核酸（HBV-DNA）研究。其中27例患者为丁型肝炎病毒（HDV）重叠感染。54例（72%）患者检测到肝内HBV-DNA，其中32例（59%）为复制型。多变量分析显示，复制型的存在与肝脏HBcAg直接相关，与肝脏HDAg呈负相关。然而，14例肝内HBV-DNA为非复制型的患者以及约一半的HDV感染患者肝脏HBcAg和/或血清HBV-DNA呈阳性，大多含量较低。多变量分析证实，无论是否存在HDV重叠感染，组织学炎症活动均与肝脏HBcAg表达密切相关。我们的结果证实了先前关于肝内HBV-DNA复制模式与肝脏HBcAg表达之间一致性以及HDV对高水平HBV复制抑制作用的研究。然而，这些结果表明，在HBV和HDV自然传播的人群中，低水平HBV复制在导致HDV感染患者肝损伤方面可能也起着重要作用。
