W. Harry Feinstone Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205-2179, USA.
Dev Comp Immunol. 2010 Jun;34(6):625-9. doi: 10.1016/j.dci.2010.01.006. Epub 2010 Jan 19.
Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We have previously demonstrated the involvement of the Toll pathway in the anti-dengue defense at 7 days after infection. In the present study, we have investigated the activity of this immune signaling pathway against different dengue virus serotypes at the early stages of infection in laboratory and field-derived mosquito strains. Our studies corroborate the importance of the Toll pathway in the anti-dengue defense repertoire at 3 days after an infectious blood meal, when new virions are released from the midgut for dissemination and infection of other mosquito tissues. These immune defenses are furthermore conserved among different Ae. aegypti strains and can act against a broad range of dengue virus serotypes.
登革病毒已成为当今世界最重要的虫媒病毒病原体之一。该病毒通过埃及伊蚊和白纹伊蚊在人群中传播。与其他虫媒病毒病原体一样，这种病毒在蚊媒中会遇到先天免疫防御，从而限制感染。我们之前已经证明 Toll 途径参与了感染后 7 天的抗登革热防御。在本研究中，我们研究了在实验室和现场来源的蚊株中感染早期，该免疫信号通路对不同登革热病毒血清型的活性。我们的研究证实了 Toll 途径在感染后 3 天从中肠释放新病毒粒子以传播和感染其他蚊组织时，在抗登革热防御库中的重要性。这些免疫防御在不同的埃及伊蚊株中是保守的，可以针对广泛的登革热病毒血清型发挥作用。
