De Rossi A, Saggioro D, Calabró M L, Cenzato R, Chieco-Bianchi L
Institute of Oncology, University of Padua, Italy.
J Acquir Immune Defic Syndr (1988). 1991;4(4):380-5.
The finding of dual HTLV-I and HIV-1 infection in populations at risk for AIDS raises the possibility that interaction between the two viruses might have clinical significance. It was shown that HTLV-I enhances HIV-1 expression, but whether HIV-1 activates HTLV-I remains to be demonstrated. To study HTLV-I behaviour following HIV-1 infection, we superinfected cells from two HTLV-I transformed cell lines with HIV-1 (strain IIIB). Viral RNA analysis indicated that HTLV-I expression in the doubly infected cells was moderately enhanced. Moreover, CAT assays in HTLV-I transformed cells transiently transfected with HTLV-I LTR-CAT disclosed higher activity in the HIV-1 superinfected cultures. This enhancement was observed only after infection with active HIV-1 virus, but not following exposure to inactivated viral particles or transfection with HIV-1 tat gene.
在艾滋病高危人群中发现同时感染人嗜T淋巴细胞病毒I型（HTLV-I）和人类免疫缺陷病毒1型（HIV-1），这增加了两种病毒之间的相互作用可能具有临床意义的可能性。研究表明，HTLV-I可增强HIV-1的表达，但HIV-1是否激活HTLV-I仍有待证实。为了研究HIV-1感染后HTLV-I的行为，我们用HIV-1（IIIB株）对两个HTLV-I转化细胞系的细胞进行了超感染。病毒RNA分析表明，双重感染细胞中HTLV-I的表达适度增强。此外，用HTLV-I长末端重复序列-氯霉素乙酰转移酶（HTLV-I LTR-CAT）瞬时转染的HTLV-I转化细胞中的CAT分析显示，在HIV-1超感染培养物中活性更高。这种增强仅在感染活性HIV-1病毒后观察到，而在暴露于灭活病毒颗粒或用HIV-1反式激活因子（tat）基因转染后未观察到。
