de Araujo A C, Casseb J S, Neitzert E, de Souza M L, Mammano F, Del Mistro A, De Rossi A, Chieco-Bianchi L
Department of Immunology, Instituto Adolfo Lutz, Sao Paulo, Brazil.
Eur J Epidemiol. 1994 Apr;10(2):165-71. doi: 10.1007/BF01730366.
To estimate the presence of, and the risk factors for HTLV-I and HTLV-II infections among HIV-1 infected subjects in Sao Paulo, Brazil, a serosurvey was performed in 471 HIV-1 infected patients, including 216 intravenous drug addicts (IVDA), 229 homosexual/bisexual men, and 26 with other risk factors. Serum samples were screened for HTLV seroreactivity by ELISA; reactive samples were analyzed by Western Blot (WB), using whole HTLV-I lysate as antigen. To confirm and discriminate HTLV-I and HTLV-II infections, sera presenting any bands on WB were further analyzed by a WB containing recombinant HTLV-I and HTLV-II proteins (WB 2.3), and by enzyme immunoassays using synthetic peptides specific for envelope proteins (Synth-EIA). In 22 cases, cell samples were available for polymerase chain reaction (PCR) studies. On WB, 114 sera were reactive and, of these, 37 and 25 were concordantly positive on both WB 2.3 and Synth-EIA procedures for HTLV-I and HTLV-II specific antibodies, respectively; 37 specimens were negative on both assays, and 15 gave discordant or indeterminate results. PCR findings confirmed concordant results obtained in the discriminatory serological assays. The prevalence rates of HTLV-I and HTLV-II infections were 15.3% and 11.1% in IVDA, and 0.9% and 0.4% in homosexual/bisexual men, respectively. No case of HTLV-I/HTLV-II co-infection was found.
为评估巴西圣保罗市HIV-1感染人群中HTLV-I和HTLV-II感染的存在情况及危险因素，对471例HIV-1感染患者进行了血清学调查，其中包括216名静脉注射吸毒者（IVDA）、229名同性恋/双性恋男性以及26名具有其他危险因素的患者。通过酶联免疫吸附测定（ELISA）对血清样本进行HTLV血清反应性筛查；对反应性样本采用全HTLV-I裂解物作为抗原，通过蛋白质印迹法（WB）进行分析。为确认和区分HTLV-I和HTLV-II感染，对在WB上出现任何条带的血清，进一步采用包含重组HTLV-I和HTLV-II蛋白的WB（WB 2.3）以及使用包膜蛋白特异性合成肽的酶免疫测定法（合成肽酶免疫测定，Synth-EIA）进行分析。在22例病例中，可获得细胞样本用于聚合酶链反应（PCR）研究。在WB上，114份血清呈反应性，其中分别有37份和25份在针对HTLV-I和HTLV-II特异性抗体的WB 2.3和Synth-EIA检测中均呈一致阳性；37份样本在两种检测中均为阴性，15份样本结果不一致或不确定。PCR结果证实了在鉴别血清学检测中获得的一致结果。IVDA中HTLV-I和HTLV-II感染的患病率分别为15.3%和11.1%，同性恋/双性恋男性中的患病率分别为0.9%和0.4%。未发现HTLV-I/HTLV-II合并感染病例。
