HSV-1 gK 免疫小鼠角膜瘢痕恶化与眼感染小鼠角膜中 CD8+CD25+T 细胞的升高相关。
Exacerbation of corneal scarring in HSV-1 gK-immunized mice correlates with elevation of CD8+CD25+ T cells in corneas of ocularly infected mice.
机构信息
Center for Neurobiology and Vaccine Development, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA; Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA.
Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA.
出版信息
Virology. 2010 Mar 30;399(1):11-22. doi: 10.1016/j.virol.2009.12.011. Epub 2010 Jan 18.
Abstract
We have shown previously that exacerbation of corneal scarring (CS) in HSV-1 glycoprotein K (gK) immunized mice was associated with CD8+ T cells. In this study, we investigated the type and the nature of the immune responses that are involved in the exacerbation of CS in gK-immunized animals. BALB/c mice were vaccinated with baculovirus expressed gK, gD, or mock-immunized. Twenty-one days after the third immunization, mice were ocularly infected with 2 x 10(5) PFU/eye of virulent HSV-1 strain McKrae. Infiltration of the cornea by CD4+, CD8+, CD25+, CD4+CD25+, CD8+CD25+, CD19+, CD40+, CD40L+, CD62L+, CD95+, B7-1+, B7-2+, MHC-I+, and MHC-II+ cells was monitored by immunohistochemistry, qRT-PCR and FACS at various times post-infection (PI). This study demonstrated for the first time that the presence of CD8+CD25+ T cells in the cornea is correlated with exacerbation of CS in the gK-immunized group.
摘要
我们之前已经表明，在 HSV-1 糖蛋白 K（gK）免疫小鼠中，角膜瘢痕（CS）的恶化与 CD8+T 细胞有关。在这项研究中，我们研究了 gK 免疫动物 CS 恶化所涉及的免疫反应的类型和性质。用杆状病毒表达的 gK、gD 或模拟免疫接种 BALB/c 小鼠。第三次免疫后 21 天，用 2×10(5)PFU/眼的强毒 HSV-1 McKrae 株眼内感染小鼠。通过免疫组织化学、qRT-PCR 和 FACS 在感染后不同时间监测角膜浸润的 CD4+、CD8+、CD25+、CD4+CD25+、CD8+CD25+、CD19+、CD40+、CD40L+、CD62L+、CD95+、B7-1+、B7-2+、MHC-I+和 MHC-II+细胞。这项研究首次表明，角膜中 CD8+CD25+T 细胞的存在与 gK 免疫组 CS 恶化相关。
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