Immunalysis Corporation, Pomona, CA 91767, USA.
Forensic Sci Int. 2010 Mar 20;196(1-3):70-3. doi: 10.1016/j.forsciint.2009.12.025. Epub 2010 Jan 15.
A semi-quantitative analytical screening procedure for the determination of cocaine, amphetamines, opiates, and delta-9-tetrahydrocannabinol in hair has been developed. The procedure employs an aqueous extraction buffer, uses only 10mg of hair, requires 2h of incubation for the extraction to occur, and multiple drug classes can be screened using enzyme linked immunosorbent assays. Hair calibration standards were prepared around the recommended cut-off concentrations of the Society of Hair Testing. All drug classes showed excellent linearity over the concentration range tested, indicating that immunochemical screening can be used in a semi-quantitative mode for hair analysis using an aqueous buffer, rapid extraction and a small amount of hair.
已经开发出一种用于在头发中测定可卡因、苯丙胺、阿片类药物和大麻酚的半定量分析筛选程序。该程序采用水性提取缓冲液，仅使用 10mg 的头发，需要 2 小时的孵育时间才能进行提取，并且可以使用酶联免疫吸附测定法同时筛选多种药物类别。头发校准标准是围绕头发测试协会推荐的截止浓度来制备的。所有药物类别在测试的浓度范围内均表现出极好的线性，表明可以在半定量模式下使用水性缓冲液、快速提取和少量头发进行免疫化学筛选，用于头发分析。
