Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, P. R. Chin.
J Surg Res. 2011 May 15;167(2):258-66. doi: 10.1016/j.jss.2009.08.006. Epub 2009 Sep 9.
Id (inhibitor of differentiation/DNA binding)-1 and -3 are involved in neoangiogenesis; they antagonize basic helix-loop-helix proteins, inhibit differentiation, and enhance cell proliferation. The aim of this study was to investigate Id-1 and -3 expression in gastric tumors and their clinical relevance in gastric cancer.
We investigated Id-1 and Id-3 expression in gastric cancer samples by immunohistochemistry and Western blotting, and further analyzed the relationship between expression of Id-1 and Id-3 and clinicopathologic characteristics.
Expression of Id-1 and -3 was found significantly more often in gastric cancers than in matched adjacent nonmalignant tissues. Cancer samples with poor or moderate histologic differentiation showed significantly stronger Id-1 and -3 expression than cancer samples with high differentiation. In cancer samples, strong or moderate expression of Id-3, but not Id-1, was a strong independent predictor for shorter overall survival in multivariate analysis.
The level of Id-1 and -3 protein expression was associated with the malignant potential of gastric tumors. In cancer samples, stronger Id-1 and -3 expression is associated with poor differentiation and more aggressive behavior of tumor cells, resulting in poor clinical outcome. Consequently, Id-3 might be used to independently predict survival of patients with gastric cancer.
Id（分化/DNA 结合抑制剂）-1 和 -3 参与新生血管形成；它们拮抗碱性螺旋-环-螺旋蛋白，抑制分化，增强细胞增殖。本研究旨在探讨 Id-1 和 -3 在胃癌中的表达及其在胃癌中的临床相关性。
我们通过免疫组织化学和 Western blot 检测了胃癌样本中 Id-1 和 Id-3 的表达，并进一步分析了 Id-1 和 Id-3 表达与临床病理特征之间的关系。
与匹配的非恶性组织相比，Id-1 和 -3 在胃癌中的表达明显更为常见。组织学分化差或中等的癌症样本的 Id-1 和 -3 表达明显强于高分化的癌症样本。在癌症样本中，Id-3 的强或中度表达，而不是 Id-1 的表达，是多变量分析中总生存期较短的一个强独立预测因子。
Id-1 和 -3 蛋白表达水平与胃肿瘤的恶性潜能相关。在癌症样本中，Id-1 和 -3 的表达越强，与肿瘤细胞分化越差和侵袭性行为越活跃相关，导致临床结局较差。因此，Id-3 可能被用来独立预测胃癌患者的生存。
