Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands.
Plant Signal Behav. 2010 Mar;5(3):271-4. doi: 10.4161/psb.5.3.10623. Epub 2010 Mar 13.
One of the strategies that plants employ to defend themselves against herbivore attack is the induced production of carnivore-attracting volatiles. Using elicitors and inhibitors of different steps of the signal-transduction pathways can improve our understanding of the mechanisms underlying induced plant defenses. For instance, we recently showed that application of jasmonic acid, a key hormone in the octadecanoid pathway involved in herbivore-induced defense, to Brassica oleracea affects gene expression, hormone levels, and volatile emission, as well as oviposition by herbivores and host location behavior by parasitoids. Such defense responses vary with the dose of the elicitor and with time since application. This addendum describes how the use of inhibitors, in addition to the use of elicitors like jasmonic acid, can be applied in bio-assays to investigate the role of signal-transduction pathways involved in induced plant defense. We show how inhibition of different steps of the octadecanoid pathway affects host location behavior by parasitoids.
植物抵御草食性动物攻击的策略之一是诱导产生吸引肉食性动物的挥发物。使用不同信号转导途径的诱导剂和抑制剂可以帮助我们更好地理解诱导植物防御的机制。例如，我们最近表明，应用茉莉酸（一种参与草食性诱导防御的十八碳烯酸途径中的关键激素）到甘蓝型油菜中会影响基因表达、激素水平和挥发物排放，以及草食性动物的产卵和寄生性天敌的寄主定位行为。这种防御反应随着诱导剂剂量和应用后时间的变化而变化。本附录描述了如何在生物测定中除了使用茉莉酸等诱导剂外，还可以使用抑制剂来研究参与诱导植物防御的信号转导途径的作用。我们展示了抑制十八碳烯酸途径的不同步骤如何影响寄生性天敌的寄主定位行为。
