Karlsruhe Institute of Technology, Karlsruhe, Germany.
J Exp Bot. 2012 Mar;63(5):2127-39. doi: 10.1093/jxb/err426. Epub 2012 Jan 5.
Salt stress is a major constraint for many crop plants, such as the moderately salt-sensitive economically important fruit crop grapevine. Plants have evolved different strategies for protection against salinity and drought. Jasmonate signalling is a central element of both biotic and abiotic stress responses. To discriminate stress quality, there must be cross-talk with parallel signal chains. Using two grapevine cell lines differing in salt tolerance, the response of jasmonate ZIM/tify-domain (JAZ/TIFY) proteins (negative regulators of jasmonate signalling), a marker for salt adaptation Na(+)/H(+) EXCHANGER (NHX1), and markers for biotic defence STILBENE SYNTHASE (StSy) and RESVERATROL SYNTHASE (RS) were analysed. It is shown that salt stress signalling shares several events with biotic defence including activity of a gadolinium-sensitive calcium influx channel (monitored by apoplastic alkalinization) and transient induction of JAZ/TIFY transcripts. Exogenous jasmonate can rescue growth in the salt-sensitive cell line. Suppression of jasmonate signalling by phenidone or aspirin blocks the induction of JAZ/TIFY transcripts. The rapid induction of RS and StSy characteristic for biotic defence in grapevine is strongly delayed in response to salt stress. In the salt-tolerant line, NHX1 is induced and the formation of reactive oxygen species, monitored as stress markers in the sensitive cell line, is suppressed. The data are discussed in terms of a model where salt stress signalling acts as a default pathway whose readout is modulated by a parallel signal chain triggered by biotic factors downstream of jasmonate signalling.
盐胁迫是许多作物的主要限制因素，如中度耐盐的经济重要水果作物葡萄。植物已经进化出不同的策略来抵御盐胁迫和干旱。茉莉酸信号是生物和非生物胁迫反应的核心要素。为了区分胁迫质量，必须与平行信号链进行交叉对话。使用耐盐性不同的两种葡萄细胞系，分析了茉莉酸 ZIM/tify 结构域（JAZ/TIFY）蛋白（茉莉酸信号的负调节剂）、盐适应 Na(+)/H(+) EXCHANGER（NHX1）的标记物和生物防御标记物 STILBENE SYNTHASE（StSy）和 RESVERATROL SYNTHASE（RS）的反应。结果表明，盐胁迫信号与生物防御共享几个事件，包括活性镧系元素敏感钙流入通道（通过质外体碱化监测）和 JAZ/TIFY 转录物的瞬时诱导。外源茉莉酸可以挽救敏感细胞系的生长。用苯并二氮杂卓或阿司匹林抑制茉莉酸信号会阻止 JAZ/TIFY 转录物的诱导。葡萄中生物防御特征的 RS 和 StSy 的快速诱导强烈延迟了对盐胁迫的反应。在耐盐系中，NHX1 被诱导，活性氧的形成（在敏感细胞系中作为应激标记物监测）被抑制。这些数据根据一种模型进行了讨论，其中盐胁迫信号作为一种默认途径，其读出由茉莉酸信号下游的生物因子触发的平行信号链调制。
