Kunugiza Y, Tomita T, Moritomo H, Yoshikawa H
Department of Orthopaedic Surgery, Graduate School of Medicine, Osaka University, Japan.
J Wound Care. 2010 Jan;19(1):10-4. doi: 10.12968/jowc.2010.19.1.46093.
Hydrocellular foam dressings are characterised by their ability to create a moist wound healing environment and absorb high amounts of exudate. In this study, we examined the effect of a hydrocellular foam dressing on the healing of rat excisional wounds.
One full-thickness wound was made on the back of rats and wounds were treated with hydrocellular foam dressing or gauze dressing. To examine the histology of the wound, haematoxylin and eosin (HE) staining was performed. Gene expression of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF) and hypoxia inducible factor-1 alpha (HIF-1a) in granulation tissue was examined by real-time reverse transcription polymerase chain reaction (real-time RT-PCR). VEGF protein was measured by ELISA.
The hydrocellular foam dressing promoted formation of granulation tissue and significantly promoted wound closure. A scab formed on the surface of granulation tissue in the gauze-treated wounds, which disturbed epithelialisation. In addition, the expression of VEGF and mRNA was higher in the gauze-treated wounds.
These results supports the hypothesis that gauze encourages scab formation, which impairs epithelialisation and increases VEGF expression. In contrast, hydrocellular foam dressing accelerates epithelialisation and new vessel formation in granulation tissue.
水凝胶泡沫敷料的特点是能够营造湿润的伤口愈合环境并吸收大量渗出液。在本研究中，我们检测了水凝胶泡沫敷料对大鼠切除伤口愈合的影响。
在大鼠背部制造一个全层伤口，伤口分别用水凝胶泡沫敷料或纱布敷料处理。为检测伤口的组织学情况，进行苏木精-伊红（HE）染色。通过实时逆转录聚合酶链反应（实时RT-PCR）检测肉芽组织中血管内皮生长因子（VEGF）、成纤维细胞生长因子（FGF）、肝细胞生长因子（HGF）和缺氧诱导因子-1α（HIF-1α）的基因表达。用酶联免疫吸附测定法（ELISA）测量VEGF蛋白。
水凝胶泡沫敷料促进了肉芽组织的形成，并显著促进了伤口闭合。在纱布处理的伤口中，肉芽组织表面形成了痂皮，这干扰了上皮形成。此外，在纱布处理的伤口中，VEGF及其mRNA的表达更高。
这些结果支持以下假设，即纱布促进痂皮形成，这会损害上皮形成并增加VEGF表达。相比之下，水凝胶泡沫敷料可加速肉芽组织中的上皮形成和新血管形成。
