Lazo O, Contreras M, Singh I
Department of Pediatrics, Medical University of South Carolina, Charleston 29425.
Mol Cell Biochem. 1991 Feb 2;100(2):159-67. doi: 10.1007/BF00234165.
The effect of ciprofibrate, a hypolipidemic drug, was examined in the metabolism of palmitic (C16:0) and lignoceric (C24:0) acids in rat liver. Ciprofibrate is a peroxisomal proliferating drug which increases the number of peroxisomes. The palmitoyl-CoA ligase activity in peroxisomes, mitochondria and microsomes from ciprofibrate treated liver was 3.2, 1.9 and 1.5-fold higher respectively and the activity for oxidation of palmitic acid in peroxisomes and mitochondria was 8.5 and 2.3-fold higher respectively. Similarly, ciprofibrate had a higher effect on the metabolism of lignoceric acid. Treatment with ciprofibrate increased lignoceroyl-CoA ligase activity in peroxisomes, mitochondria and microsomes by 5.3, 3.3 and 2.3-fold respectively and that of oxidation of lignoceric acid was increased in peroxisomes and mitochondria by 13.4 and 2.3-fold respectively. The peroxisomal rates of oxidation of palmitic acid (8.5-fold) and lignoceric acid (13.4-fold) were increased to a different degree by ciprofibrate treatment. This differential effect of ciprofibrate suggests that different enzymes may be responsible for the oxidation of fatty acids of different chain length, at least at one or more step(s) of the peroxisomal fatty acid beta-oxidation pathway.
研究了降血脂药物环丙贝特对大鼠肝脏中棕榈酸（C16:0）和木蜡酸（C24:0）代谢的影响。环丙贝特是一种过氧化物酶体增殖药物，可增加过氧化物酶体的数量。用环丙贝特处理的肝脏中，过氧化物酶体、线粒体和微粒体中的棕榈酰辅酶A连接酶活性分别提高了3.2倍、1.9倍和1.5倍，过氧化物酶体和线粒体中棕榈酸的氧化活性分别提高了8.5倍和2.3倍。同样，环丙贝特对木蜡酸的代谢也有更高的影响。用环丙贝特处理后，过氧化物酶体、线粒体和微粒体中的木蜡酰辅酶A连接酶活性分别提高了5.3倍、3.3倍和2.3倍，过氧化物酶体和线粒体中木蜡酸的氧化活性分别提高了13.4倍和2.3倍。环丙贝特处理使棕榈酸（8.5倍）和木蜡酸（13.4倍）的过氧化物酶体氧化速率有不同程度的提高。环丙贝特的这种差异效应表明，不同链长脂肪酸的氧化可能由不同的酶负责，至少在过氧化物酶体脂肪酸β-氧化途径的一个或多个步骤中是这样。
