Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.
Technol Cancer Res Treat. 2010 Feb;9(1):71-6. doi: 10.1177/153303461000900108.
We developed an automatic occlusion device for remote control of tumor tissue ischemia. The device consists of a flexible cannula encasing a shape memory alloy wire with its distal end connected to surgical suture. Regional tissue occlusion was tested on both the benchtop and the animal models. In the benchtop test, the occlusion device introduced quantitative and reproducible changes of blood flow in a tissue simulating phantom embedding a vessel simulator. In the animal test, the device generated a cyclic pattern of reversible ischemia in the right hinder leg tissue of a black male C57BL/6 mouse. We also developed a multimodal detector that integrates near infrared spectroscopy and electron paramagnetic resonance spectroscopy for continuous monitoring of tumor tissue oxygenation, blood content, and oxygen tension changes. The multimodal detector was tested on a cancer xenograft nude mouse undergoing reversible tumor ischemia. The automatic occlusion device and the multimodal detector can be potentially integrated for closed-loop feedback control of tumor tissue ischemia. Such an integrated occlusion device may be used in multiple clinical applications such as regional hypoperfusion control in tumor resection surgeries and thermal ablation processes. In addition, the proposed occlusion device can also be used as a research tool to understand tumor oxygen transport and hemodynamic characteristics.
我们开发了一种用于远程控制肿瘤组织缺血的自动闭塞装置。该装置由一个柔性套管包裹形状记忆合金丝组成，其远端连接到手术缝线。在台架和动物模型上都进行了区域性组织闭塞测试。在台架测试中，闭塞装置在嵌入血管模拟器的组织模拟体中引入了定量且可重复的血流变化。在动物测试中，该装置在一只黑色雄性 C57BL/6 小鼠的右后腿组织中产生了可循环的可逆性缺血模式。我们还开发了一种多模态探测器，它集成了近红外光谱和电子顺磁共振光谱，用于连续监测肿瘤组织氧合、血液含量和氧分压变化。该多模态探测器在接受可逆肿瘤缺血的癌症异种移植裸鼠上进行了测试。自动闭塞装置和多模态探测器可以潜在地集成在一起，以实现肿瘤组织缺血的闭环反馈控制。这种集成的闭塞装置可用于多种临床应用，如肿瘤切除术和热消融过程中的区域性低灌注控制。此外，所提出的闭塞装置还可以用作研究工具，以了解肿瘤氧运输和血液动力学特性。
