Department of Burn and Plastic Surgery, Wuxi third People's Hospital, Wuxi, China.
Wound Repair Regen. 2010 Jan-Feb;18(1):70-9. doi: 10.1111/j.1524-475X.2009.00557.x.
In situ photopolymerized hydrogel dressings create minimally invasive methods that offer advantages over the use of preformed dressings such as conformability in any wound bed, convenience of application, and improved patient compliance and comfort. Here, we report an in situ-formed hydrogel membrane through ultraviolet cross-linking of a photocross-linkable azidobenzoic hydroxypropyl chitosan aqueous solution. The hydrogel membrane is stable, flexible, and transparent, with a bulk network structure of smoothness, integrity, and density. Fluid uptake ability, water vapor transmission rate, water retention, and bioadhesion of the thus resulted hydrogel membranes (0.1 mm thick) were determined to range from 97.0-96.3%, 2,934-2,561 g/m(2)/day, 36.69-22.94% (after 6 days), and 4.8-12.3 N/cm(2), respectively. These data indicate that the hydrogel membrane can maintain a long period of moist environment over the wound bed for enhancing reepithelialization. Specifically, these properties of the hydrogel membrane were controllable to some extent, by adjusting the substitution degree of the photoreactive azide groups. The hydrogel membrane also exhibited barrier function, as it was impermeable to bacteria but permeable to oxygen. In vitro experiments using two major skin cell types (dermal fibroblast and epidermal keratinocyte) revealed the hydrogel membrane have neither cytotoxicity nor an effect on cell proliferation. Taken together, the in situ photocross-linked azidobenzoic hydroxypropyl chitosan hydrogel membrane has a great potential in the management of wound healing and skin burn.
原位光聚合水凝胶敷料创造了微创方法，与使用预制敷料相比具有优势，例如在任何伤口床上的顺应性、应用方便性以及提高患者的依从性和舒适度。在这里，我们通过光交联可聚合叠氮苯甲酸羟丙基壳聚糖水溶液的紫外光交联报告了一种原位形成的水凝胶膜。水凝胶膜稳定、柔韧、透明，具有光滑、完整和致密的整体网络结构。所得到的水凝胶膜（0.1 毫米厚）的吸液能力、水蒸气透过率、保水率和生物粘附性分别为 97.0-96.3%、2934-2561 g/m2/天、36.69-22.94%（6 天后）和 4.8-12.3 N/cm2。这些数据表明，水凝胶膜可以在伤口床上保持长时间的湿润环境，从而促进再上皮化。具体而言，通过调整光反应性叠氮基团的取代度，可以在一定程度上控制水凝胶膜的这些性质。水凝胶膜还表现出屏障功能，因为它对细菌不可渗透，但对氧气可渗透。使用两种主要的皮肤细胞类型（真皮成纤维细胞和表皮角质形成细胞）的体外实验表明，水凝胶膜既没有细胞毒性也不会影响细胞增殖。总之，原位光交联叠氮苯甲酸羟丙基壳聚糖水凝胶膜在伤口愈合和皮肤烧伤的治疗中有很大的潜力。
