Vuković Jovana S, Perišić Srđan, Nikolić Anja, Milošević Ivan, Mirilović Milorad, Bolka Prokić Bogomir, Lužajić Božinovski Tijana
Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
Pharmaceutics. 2025 Apr 28;17(5):578. doi: 10.3390/pharmaceutics17050578.
: Efficient wound treatment embraces the management of four overlapping phases, starting with hemostasis, an immediate physiological response aimed at stopping bleeding from damaged blood vessels caused by skin injury. This paper proposes an innovative, nature-based hemostatic biomaterial designed to assist natural self-healing regenerative mechanisms. : Light, transparent, and skin-adhesive films based on κ-carrageenan, meadow polyfloral honey, and flower extract were fabricated via solution casting. Comprehensive characterization revealed the physicochemical, structural, swelling, and barrier properties and the influence of each bioactive compound utilized for film preparation. : The samples subcutaneously implanted in Wistar rats induced vascularization, deposition of collagen, and orientation of collagen fibers while being fully phagocytosed and gradually biodegraded. The rat tail-cut model demonstrated that the films significantly reduced blood loss (0.1875 ± 0.0732 g) compared to the control (0.7837 ± 0.3319 g), and hemostasis was achieved notably faster (355.75 ± 71.42 s) than in the control group (704.25 ± 85.29 s). The rat liver punch biopsy model confirmed reduced blood loss (2.8025 ± 1.5174 g) and shorter time to hemostasis (303.25 ± 77.90 s) compared to the control (3.1475 ± 1.5413 g, 383.00 ± 36.53 s). : The results indicate the great potential of the fabricated films as hemostatic wound dressings.
高效的伤口治疗涵盖四个重叠阶段的管理，始于止血，这是一种即时的生理反应，旨在阻止因皮肤损伤导致的受损血管出血。本文提出了一种创新的、基于天然物质的止血生物材料，旨在辅助自然的自我愈合再生机制。基于κ-卡拉胶、多花野蜂蜜和花提取物制备了轻质、透明且具有皮肤粘附性的薄膜，采用溶液浇铸法进行制备。全面表征揭示了其物理化学、结构、膨胀和阻隔性能以及用于薄膜制备的每种生物活性化合物的影响。皮下植入Wistar大鼠的样品诱导了血管生成、胶原蛋白沉积以及胶原蛋白纤维的定向排列，同时被完全吞噬并逐渐生物降解。大鼠断尾模型表明，与对照组（0.7837±0.3319克）相比，该薄膜显著减少了失血量（0.1875±0.0732克），并且止血速度明显更快（355.75±71.42秒），而对照组为（704.25±85.29秒）。大鼠肝脏穿刺活检模型证实，与对照组（3.1475±1.5413克，383.00±36.53秒）相比，失血量减少（2.8025±1.5174克）且止血时间更短（303.25±77.90秒）。结果表明所制备的薄膜作为止血伤口敷料具有巨大潜力。
