Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
Wound Repair Regen. 2010 Jan-Feb;18(1):132-8. doi: 10.1111/j.1524-475X.2009.00565.x.
We investigated the effects of repairing large tympanic membrane (TM) perforations in rats with a thin silk patch compared with the commonly used paper patch. We performed bilateral myringotomies of 1.8 mm in diameter on 50 adult Sprague-Dawley rats with intact TMs. The perforations in the right ears of 40 rats were treated with a silk patch, and the perforations in the left ears of the same rats were treated with a paper patch. Ten rats acted as controls. The mean healing times of the TM perforations on the silk-patch-treated ears and the paper-patch-treated ears were 7.2+/-1.48 and 9.1+/-1.11 days, respectively (control 10.38+/-1.70 days). The difference between silk-patch- and paper-patch-treated ears was statistically significant, with a mean difference of 1.9 days (0.6-4.5 days). The mean perforation closure times were significantly shorter in silk-patch- and paper-patch-treated ears than in the control animals. The endoscopic and histological findings of this study provide evidence that silk-patch treatment accelerates wound healing and shortens TM perforation closure time. We suggest that the silk patch may prove to be an effective material for repairing TM perforations in human patients in an outpatient clinical setting.
我们研究了用薄丝绸片修复大鼠大鼓膜穿孔与常用纸补片的效果。我们对 50 只成年 Sprague-Dawley 大鼠进行双侧鼓膜切开术，鼓膜完整。40 只大鼠右耳的穿孔用丝绸片治疗，同一大鼠左耳的穿孔用纸补片治疗。10 只大鼠作为对照。丝绸片治疗组和纸补片治疗组鼓膜穿孔的平均愈合时间分别为 7.2+/-1.48 天和 9.1+/-1.11 天（对照组 10.38+/-1.70 天）。丝绸片和纸补片治疗组之间的差异具有统计学意义，平均差异为 1.9 天（0.6-4.5 天）。丝绸片和纸补片治疗组的穿孔闭合时间明显短于对照组。本研究的内镜和组织学发现提供了证据，表明丝绸片治疗可加速创面愈合并缩短鼓膜穿孔闭合时间。我们建议，在门诊临床环境中，丝绸补片可能被证明是修复人类患者鼓膜穿孔的有效材料。
